Reference - Detail
| RRC ID | 28977 |
|---|---|
| Author | Caterina MJ, Milne JL, Devreotes PN. |
| Title | Mutation of the third intracellular loop of the cAMP receptor, cAR1, of Dictyostelium yields mutants impaired in multiple signaling pathways. |
| Journal | J Biol Chem |
| Abstract |
Seven-membrane span receptors transduce a wide range of signals across the plasma membrane. One member of this family, the cAMP receptor, cAR1, of Dictyostelium, mediates some responses (e.g. adenylyl cyclase activation, multicellular aggregation) which require G-proteins and others (e.g. Ca2+ influx, loss of ligand binding, cAR1 phosphorylation) which appear to be G-protein-independent. In this study, we randomly mutagenized the NH2-terminal eight amino acids of the third intracellular loop of cAR1 and examined the ability of these mutants to exhibit the three G-protein-independent responses listed above. Most mutants (classes I, II) exhibited wild-type or midly defective responses. Several mutants (class III), however, were severely impaired in all three processes but not in cAMP binding. Furthermore, these mutants failed to couple productively with G-proteins and could not replace cAR1 in a car1- cell. For these reasons, we propose that class III mutations interfere with the formation of an "active" conformation of the receptor. |
| Volume | 269(2) |
| Pages | 1523-32 |
| Published | 1994-1-14 |
| PII | S0021-9258(17)42288-5 |
| PMID | 8288619 |
| MeSH | Amino Acid Sequence Animals Base Sequence Biological Transport Calcium / metabolism Cyclic AMP / metabolism DNA Mutational Analysis Dictyostelium GTP-Binding Proteins / physiology Molecular Sequence Data Oligodeoxyribonucleotides / chemistry Phosphorylation Point Mutation Protein Structure, Tertiary Receptors, Cyclic AMP / genetics Receptors, Cyclic AMP / metabolism* Signal Transduction Structure-Activity Relationship |
| IF | 4.238 |
| Times Cited | 72 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| Total number of mentions | 0 |
| Resource | |
| Cellular slime molds | S00056 S00152 S00343 |