Reference - Detail
| RRC ID | 28992 |
|---|---|
| Author | Flick KM, Shaulsky G, Loomis WF. |
| Title | The wacA gene of Dictyostelium discoideum is a developmentally regulated member of the MIP family. |
| Journal | Gene |
| Abstract |
We isolated a cDNA from Dictyostelium discoideum that encodes a 30 kDa protein with significant similarity to members of the major intrinsic protein (MIP) family of membrane transporters. The most closely related protein in the public data bases is an aquaporin from Cicadella viridis which shows 34% identity. The cDNA was used to isolate and characterize genomic fragments carrying the Dictyostelium gene which we named wacA. Genomic probes were used to recognize wacA mRNA isolated at various stages of development. The results showed that the gene is developmentally regulated such that the mRNA first appears at 12 h of development and is retained throughout the remainder of development. In situ hybridization of whole mounts prepared at 15 h of development showed that wacA mRNA accumulates exclusively in prespore cells and is absent from prestalk cells. Although wacA expression is prespore specific, disruption of the gene by homologous recombination did not result in observable alterations in the formation of spores or their resistance to osmotic challenges. |
| Volume | 195(2) |
| Pages | 127-30 |
| Published | 1997-8-22 |
| DOI | 10.1016/s0378-1119(97)00078-4 |
| PII | S0378-1119(97)00078-4 |
| PMID | 9305756 |
| MeSH | Amino Acid Sequence Animals Aquaporins* Chromosome Mapping Cloning, Molecular DNA Probes / genetics Dictyostelium / genetics* Dictyostelium / growth & development Eye Proteins / genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Genes, Fungal Genes, Protozoan In Situ Hybridization Insecta / genetics Membrane Glycoproteins* Molecular Sequence Data Osmotic Pressure Porins / genetics* Protozoan Proteins* RNA, Messenger / genetics RNA, Messenger / metabolism Recombination, Genetic Sequence Homology, Amino Acid Spores, Fungal / genetics Spores, Fungal / growth & development Spores, Fungal / metabolism |
| IF | 2.984 |
| Times Cited | 21 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | S00256 S90374 |