| RRC ID |
2900
|
| 著者 |
Castillo AM, Gutiérrez MC, Kamekura M, Xue Y, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa A.
|
| タイトル |
Natrinema ejinorense sp. nov., isolated from a saline lake in Inner Mongolia, China.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A Gram-negative, non-motile, neutrophilic, pleomorphic and extremely halophilic archaeon, strain EJ-57T, was isolated from saline Lake Ejinor in Inner Mongolia, China. Strain EJ-57T was able to grow at 25-50 degrees C, required at least 1.8 M NaCl for growth (optimum at 3.4 M NaCl) and grew over a pH range from 6.0 to 8.5 (optimum at pH 7.0). Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. Analysis of the almost complete 16S rRNA gene sequence indicated that the isolate represented a member of the genus Natrinema in the family Halobacteriaceae. Strain EJ-57T was most closely related to Natrinema versiforme JCM 10478T (96.2% sequence similarity), Natrinema pallidum NCIMB 777T (95.9% sequence similarity), Natrinema altunense JCM 12890T (95.8% sequence similarity) and Natrinema pellirubrum NCIMB 786T (95.5 % sequence similarity). However, DNA-DNA hybridization experiments showed that strain EJ-57T was not related to these species, with levels of DNA-DNA relatedness equal to or below 39%. The major polar lipids of the isolate were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and the disulfated glycolipid S2-DGA-1. The G+C content of the genomic DNA was 64.7 mol%. Comparative analysis of phenotypic characteristics between strain EJ-57T and recognized Natrinema species supported the conclusion that EJ-57T represents a novel species within this genus, for which the name Natrinema ejinorense sp. nov. is proposed. The type strain is EJ-57T (=CECT 7144T=JCM 13890T=CGMCC 1.6202T).
|
| 巻・号 |
56(Pt 11)
|
| ページ |
2683-2687
|
| 公開日 |
2006-11-1
|
| DOI |
10.1099/ijs.0.64421-0
|
| PMID |
17082411
|
| MeSH |
Base Composition
Carbohydrate Metabolism
China
DNA, Archaeal / chemistry
DNA, Archaeal / isolation & purification
DNA, Ribosomal / chemistry
DNA, Ribosomal / isolation & purification
Gelatin / metabolism
Genes, rRNA
Halobacteriaceae / classification*
Halobacteriaceae / cytology
Halobacteriaceae / isolation & purification*
Halobacteriaceae / physiology
Hydrogen Sulfide / metabolism
Hydrogen-Ion Concentration
Lipids / analysis
Molecular Sequence Data
Movement
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S / genetics
Saline Solution, Hypertonic
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Sodium Chloride / metabolism
Starch / metabolism
Temperature
Water Microbiology*
|
| IF |
2.415
|
| 引用数 |
12
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
JCM 10478?
JCM 12890?
JCM 13890? |
