論文 - 詳細
| RRC ID | 2900 |
|---|---|
| 著者 | Castillo AM, Gutiérrez MC, Kamekura M, Xue Y, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa A. |
| タイトル | Natrinema ejinorense sp. nov., isolated from a saline lake in Inner Mongolia, China. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A Gram-negative, non-motile, neutrophilic, pleomorphic and extremely halophilic archaeon, strain EJ-57T, was isolated from saline Lake Ejinor in Inner Mongolia, China. Strain EJ-57T was able to grow at 25-50 degrees C, required at least 1.8 M NaCl for growth (optimum at 3.4 M NaCl) and grew over a pH range from 6.0 to 8.5 (optimum at pH 7.0). Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. Analysis of the almost complete 16S rRNA gene sequence indicated that the isolate represented a member of the genus Natrinema in the family Halobacteriaceae. Strain EJ-57T was most closely related to Natrinema versiforme JCM 10478T (96.2% sequence similarity), Natrinema pallidum NCIMB 777T (95.9% sequence similarity), Natrinema altunense JCM 12890T (95.8% sequence similarity) and Natrinema pellirubrum NCIMB 786T (95.5 % sequence similarity). However, DNA-DNA hybridization experiments showed that strain EJ-57T was not related to these species, with levels of DNA-DNA relatedness equal to or below 39%. The major polar lipids of the isolate were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and the disulfated glycolipid S2-DGA-1. The G+C content of the genomic DNA was 64.7 mol%. Comparative analysis of phenotypic characteristics between strain EJ-57T and recognized Natrinema species supported the conclusion that EJ-57T represents a novel species within this genus, for which the name Natrinema ejinorense sp. nov. is proposed. The type strain is EJ-57T (=CECT 7144T=JCM 13890T=CGMCC 1.6202T). |
| 巻・号 | 56(Pt 11) |
| ページ | 2683-2687 |
| 公開日 | 2006-11-1 |
| DOI | 10.1099/ijs.0.64421-0 |
| PMID | 17082411 |
| MeSH | Base Composition Carbohydrate Metabolism China DNA, Archaeal / chemistry DNA, Archaeal / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Gelatin / metabolism Genes, rRNA Halobacteriaceae / classification* Halobacteriaceae / cytology Halobacteriaceae / isolation & purification* Halobacteriaceae / physiology Hydrogen Sulfide / metabolism Hydrogen-Ion Concentration Lipids / analysis Molecular Sequence Data Movement Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S / genetics Saline Solution, Hypertonic Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Starch / metabolism Temperature Water Microbiology* |
| IF | 2.415 |
| 引用数 | 12 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | JCM 10478? JCM 12890? JCM 13890? |