| RRC ID |
29006
|
| 著者 |
Chae SC, Inazu Y, Amagai A, Maeda Y.
|
| タイトル |
Underexpression of a novel gene, dia2, impairs the transition of Dictyostelium cells from growth to differentiation.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
In Dictyostelium discoideum Ax-2 cells, a specific point (PS-point) in the cell cycle from which they initiate differentiation in response to starvation has been specified. Using synchronized Ax-2 cells and the differential display method, a novel gene (differentiation-associated gene 2; dia2) was isolated as one of the genes expressed specifically during the shift of Ax-2 cells from growth to differentiation. The dia2 gene codes a lysine- and leucine-rich protein with a predicted molecular mass of 16.9 kDa. Northern blot analysis has shown that the dia2 mRNA, of 0.7 kb, accumulates in differentiating cells starved just before the PS-point, while there is no detectable expression in vegetatively growing cells. Antisense-mediated gene inactivation of dia2 greatly inhibited the progress of differentiation, presumably through the reduced expression of cAMP receptor 1 (car1). Thus, the DIA2 expression was suggested to have an essential role in the initiation of differentiation, closely relating to the cAMP signaling system.
|
| 巻・号 |
252(1)
|
| ページ |
278-83
|
| 公開日 |
1998-11-9
|
| DOI |
10.1006/bbrc.1998.9461
|
| PII |
S0006-291X(98)99461-X
|
| PMID |
9813183
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cell Cycle / genetics*
Cell Differentiation
DNA Primers
Dictyostelium / cytology
Dictyostelium / genetics*
Dictyostelium / growth & development
Gene Deletion
Gene Expression Regulation, Developmental
Genes, Protozoan*
Molecular Sequence Data
Molecular Weight
Polymerase Chain Reaction
Promoter Regions, Genetic
Protozoan Proteins / biosynthesis
Protozoan Proteins / chemistry
Protozoan Proteins / genetics*
|
| IF |
2.985
|
| 引用数 |
16
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
S90293 |
