論文 - 詳細
| RRC ID | 29029 |
|---|---|
| 著者 | van Es S, Weening KE, Devreotes PN. |
| タイトル | The protein kinase YakA regulates g-protein-linked signaling responses during growth and development of Dictyostelium. |
| ジャーナル | J Biol Chem |
| Abstract |
A genetic screen for Dictyostelium mutants that phenotypically resemble cells lacking the G-protein beta-subunit yielded the protein kinase YakA. Like gbeta-null cells, yakA-null cells fail to enter development and display slow growth on bacterial lawns. We created a temperature-sensitive yakA mutant and showed that YakA activity is required not only at the onset but also during development. The yakA-null cells have strong defects in folic acid-induced responses, such as actin polymerization and cGMP accumulation, indicating that they play a role in G-protein-mediated signaling responses. We propose that YakA acts downstream of G-proteins, because cAMP receptors still couple to G-proteins in the yakA mutant. In addition, the previously observed growth arrest induced by overexpression of YakA also occurs in gbeta mutants. We localized YakA-GFP to the cytosol suggesting that YakA may be a functional homolog of its mammalian counterparts Dyrk2 and Dyrk3, a subclass of dual-specificity Yak-related kinases (Dyrk) with unknown function. |
| 巻・号 | 276(33) |
| ページ | 30761-5 |
| 公開日 | 2001-8-17 |
| DOI | 10.1074/jbc.M103365200 |
| PII | S0021-9258(20)80218-X |
| PMID | 11410593 |
| MeSH | Animals Cyclic AMP-Dependent Protein Kinases / physiology Cytosol / chemistry Dictyostelium / growth & development* Folic Acid / pharmacology GTP-Binding Proteins / physiology* Protein Kinases / physiology* Signal Transduction |
| IF | 4.238 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00138 |