論文 - 詳細
| RRC ID | 29036 |
|---|---|
| 著者 | Martens H, Novotny J, Oberstrass J, Steck TL, Postlethwait P, Nellen W. |
| タイトル | RNAi in Dictyostelium: the role of RNA-directed RNA polymerases and double-stranded RNase. |
| ジャーナル | Mol Biol Cell |
| Abstract |
We show that in Dictyostelium discoideum an endogenous gene as well as a transgene can be silenced by introduction of a gene construct that is transcribed into a hairpin RNA. Gene silencing was accompanied by the appearance of sequence-specific RNA about 23mers and seemed to have a limited capacity. The three Dictyostelium homologues of the RNA-directed RNA polymerase (RrpA, RrpB, and DosA) all contain an N-terminal helicase domain homologous to the one in the dicer nuclease, suggesting exon shuffling between RNA-directed RNA polymerase and the dicer homologue. Only the knock-out of rrpA resulted in a loss of the hairpin RNA effect and simultaneously in a loss of detectable about 23mers. However, about 23mers were still generated by the Dictyostelium dsRNase in vitro with extracts from rrpA(-), rrpB(-), and DosA(-) cells. Both RrpA and a target gene were required for production of detectable amounts of about 23mers, suggesting that target sequences are involved in about 23mer amplification. |
| 巻・号 | 13(2) |
| ページ | 445-53 |
| 公開日 | 2002-2-1 |
| DOI | 10.1091/mbc.01-04-0211 |
| PMID | 11854403 |
| PMC | PMC65640 |
| MeSH | Animals Dictyostelium / genetics* Discoidins Gene Silencing* Lectins* Protozoan Proteins / genetics RNA, Double-Stranded / genetics* RNA, Double-Stranded / physiology RNA-Dependent RNA Polymerase / genetics* Ribonucleases / genetics* |
| IF | 3.791 |
| 引用数 | 123 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 33 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00457 |