論文 - 詳細
| RRC ID | 29039 |
|---|---|
| 著者 | Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL. |
| タイトル | Identification of four candidate cGMP targets in Dictyostelium. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
In Dictyostelium, a transient increase in intracellular cGMP is important for cytoskeletal rearrangements during chemotaxis. There must be cGMP-binding proteins in Dictyostelium that regulate key cytoskeletal components after treatment with chemoattractants, but to date, no such proteins have been identified. Using a bioinformatics approach, we have found four candidate cGMP-binding proteins (GbpA-D). GbpA and -B have two tandem cGMP-binding sites downstream of a metallo beta-lactamase domain, a superfamily that includes cAMP phosphodiesterases. GbpC contains the following nine domains (in order): leucine-rich repeats, Ras, MEK kinase, Ras guanine nucleotide exchange factor N-terminal (RasGEF-N), DEP, RasGEF, cGMP-binding, GRAM, and a second cGMP-binding domain. GbpD is related to GbpC, but is much shorter; it begins with the RasGEF-N domain, and lacks the DEP domain. Disruption of the gbpC gene results in loss of all high-affinity cGMP-binding activity present in the soluble cellular fraction. GbpC mRNA levels increase dramatically 8 h after starvation is initiated. GbpA, -B, and -D mRNA levels show less dramatic changes, with gbpA mRNA levels highest 4 h into starvation, gbpB mRNA levels highest in vegetative cells, and gbpD levels highest at 8 h. The identification of these genes is the first step in a molecular approach to studying downstream effects of cGMP signaling in Dictyostelium. |
| 巻・号 | 99(10) |
| ページ | 6749-54 |
| 公開日 | 2002-5-14 |
| DOI | 10.1073/pnas.102167299 |
| PII | 99/10/6749 |
| PMID | 12011437 |
| PMC | PMC124474 |
| MeSH | Amino Acid Sequence Animals Carrier Proteins / classification Carrier Proteins / genetics* Cyclic GMP / metabolism* Dictyostelium / genetics* Dictyostelium / growth & development Gene Expression Intracellular Signaling Peptides and Proteins* Molecular Sequence Data Protozoan Proteins / classification Protozoan Proteins / genetics* Sequence Homology, Amino Acid |
| IF | 9.412 |
| 引用数 | 66 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00008 S00010 S00142 S00143 S00338 S00339 S00340 |