RRC ID 29039
Author Goldberg JM, Bosgraaf L, Van Haastert PJ, Smith JL.
Title Identification of four candidate cGMP targets in Dictyostelium.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract In Dictyostelium, a transient increase in intracellular cGMP is important for cytoskeletal rearrangements during chemotaxis. There must be cGMP-binding proteins in Dictyostelium that regulate key cytoskeletal components after treatment with chemoattractants, but to date, no such proteins have been identified. Using a bioinformatics approach, we have found four candidate cGMP-binding proteins (GbpA-D). GbpA and -B have two tandem cGMP-binding sites downstream of a metallo beta-lactamase domain, a superfamily that includes cAMP phosphodiesterases. GbpC contains the following nine domains (in order): leucine-rich repeats, Ras, MEK kinase, Ras guanine nucleotide exchange factor N-terminal (RasGEF-N), DEP, RasGEF, cGMP-binding, GRAM, and a second cGMP-binding domain. GbpD is related to GbpC, but is much shorter; it begins with the RasGEF-N domain, and lacks the DEP domain. Disruption of the gbpC gene results in loss of all high-affinity cGMP-binding activity present in the soluble cellular fraction. GbpC mRNA levels increase dramatically 8 h after starvation is initiated. GbpA, -B, and -D mRNA levels show less dramatic changes, with gbpA mRNA levels highest 4 h into starvation, gbpB mRNA levels highest in vegetative cells, and gbpD levels highest at 8 h. The identification of these genes is the first step in a molecular approach to studying downstream effects of cGMP signaling in Dictyostelium.
Volume 99(10)
Pages 6749-54
Published 2002-5-14
DOI 10.1073/pnas.102167299
PII 99/10/6749
PMID 12011437
PMC PMC124474
MeSH Amino Acid Sequence Animals Carrier Proteins / classification Carrier Proteins / genetics* Cyclic GMP / metabolism* Dictyostelium / genetics* Dictyostelium / growth & development Gene Expression Intracellular Signaling Peptides and Proteins* Molecular Sequence Data Protozoan Proteins / classification Protozoan Proteins / genetics* Sequence Homology, Amino Acid
IF 9.58
Times Cited 66
Cellular slime molds S00008 S00010 S00142 S00143 S00338 S00339 S00340