Reference - Detail
| RRC ID | 29043 |
|---|---|
| Author | Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, Van Haastert PJ. |
| Title | A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium. |
| Journal | EMBO J |
| Abstract |
Chemotactic stimulation of Dictyostelium cells results in a transient increase in cGMP levels, and transient phosphorylation of myosin II heavy and regulatory light chains. In Dictyostelium, two guanylyl cyclases and four candidate cGMP-binding proteins (GbpA- GbpD) are implicated in cGMP signalling. GbpA and GbpB are homologous proteins with a Zn2+-hydrolase domain. A double gbpA/gbpB gene disruption leads to a reduction of cGMP-phosphodiesterase activity and a 10-fold increase of basal and stimulated cGMP levels. Chemotaxis in gbpA(-)B(-) cells is associated with increased myosin II phosphorylation compared with wild-type cells; formation of lateral pseudopodia is suppressed resulting in enhanced chemotaxis. GbpC is homologous to GbpD, and contains Ras, MAPKKK and Ras-GEF domains. Inactivation of the gbp genes indicates that only GbpC harbours high affinity cGMP-binding activity. Myosin phosphorylation, assembly of myosin in the cytoskeleton as well as chemotaxis are severely impaired in mutants lacking GbpC and GbpD, or mutants lacking both guanylyl cyclases. Thus, a novel cGMP signalling cascade is critical for chemotaxis in Dictyostelium, and plays a major role in myosin II regulation during this process. |
| Volume | 21(17) |
| Pages | 4560-70 |
| Published | 2002-9-2 |
| DOI | 10.1093/emboj/cdf438 |
| PMID | 12198158 |
| PMC | PMC126179 |
| MeSH | 3',5'-Cyclic-AMP Phosphodiesterases / genetics 3',5'-Cyclic-AMP Phosphodiesterases / metabolism Animals Carrier Proteins / metabolism Chemotaxis / physiology Cyclic GMP / physiology* Cytoskeleton / metabolism Dictyostelium / drug effects Dictyostelium / genetics Dictyostelium / physiology* Guanylate Cyclase / genetics Guanylate Cyclase / metabolism Intracellular Signaling Peptides and Proteins* Myosin Type II / metabolism* Phosphorylation Protein Processing, Post-Translational Protozoan Proteins / genetics Protozoan Proteins / metabolism* Second Messenger Systems / physiology* |
| IF | 9.889 |
| Times Cited | 115 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | S00008 S00010 S00142 S00143 S00338 S00339 S00340 |