| RRC ID |
29049
|
| 著者 |
Yokoyama K, Hiratuka Y, Akimaru E, Hirose K, Uyeda TQ, Suzuki M.
|
| タイトル |
Design and functional analysis of actomyosin motor domain chimera proteins.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
To gain more structural and functional information on the actomyosin complexes, we have engineered chimera proteins carrying the entire Dictyostelium actin in the loop 2 sequence of the motor domain of Dictyostelium myosin II. Although the chimera proteins were unable to polymerize by themselves, addition of skeletal actin promoted polymerization. Electron microscopic observation demonstrated that the chimera proteins were incorporated into actin filaments, when copolymerized with skeletal actin. Copolymerization with skeletal actin greatly enhanced the MgATPase, while the chimera proteins without added skeletal actin hydrolyzed ATP at a very low rate. These results indicate that the actin part and the motor domain part of the chimera proteins are correctly folded, but the chimera proteins are structurally stressed so that efficient polymerization is inhibited.
|
| 巻・号 |
299(5)
|
| ページ |
825-31
|
| 公開日 |
2002-12-20
|
| DOI |
10.1016/s0006-291x(02)02758-4
|
| PII |
S0006291X02027584
|
| PMID |
12470653
|
| MeSH |
Actin Cytoskeleton / ultrastructure
Actins / genetics*
Actomyosin / chemistry
Actomyosin / genetics*
Actomyosin / physiology
Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism
Amino Acid Sequence
Animals
Cell Line
Chickens
Dictyostelium
Molecular Motor Proteins / chemistry
Molecular Motor Proteins / genetics*
Molecular Motor Proteins / physiology
Molecular Sequence Data
Myosin Type II / genetics*
Protein Engineering
Protein Structure, Tertiary
Recombinant Fusion Proteins / analysis
Recombinant Fusion Proteins / isolation & purification
Recombinant Fusion Proteins / ultrastructure
Sequence Alignment
|
| IF |
2.985
|
| 引用数 |
8
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
S00445
G90097 |
