Reference - Detail
| RRC ID | 29061 |
|---|---|
| Author | Tsujioka M, Yamamoto T, Thompson CR, Kay RR, Maeda M. |
| Title | Novel development rescuing factors (DRFs) secreted by the developing Dictyostelium cells, that are involved in the restoration of a mutant lacking MAP-kinase ERK2. |
| Journal | Zoolog Sci |
| Abstract |
We found novel development rescuing factors (DRFs) secreted from developing Dictyostelium cells, by using a mutant (erkB-) which is missing MAP-kinase ERK2 as a test strain for bioassay. The mutant erkB- fails to undergo multicellular morphogenesis due to impaired cAMP signaling. However, such developmental defect can be restored by the presence of low-molecular weight DRFs that are secreted from developing wild-type cells. We previously showed that DIF-1 (Differentiation-Inducing Factor 1 for stalk cells) possesses this activity, indicating a newly discovered role of DIF-1. Surprisingly, however, the mutant dmtA-, which is incapable of DIF-1 synthesis still exerts a strong inducing activity of the multicellular morphogenesis of erkB-. After analysis of HPLC fractions of conditioned media prepared from both wild type Ax2 and dmtA- strains revealed that both strains secrete at least two novel DRF activities with DIF-like mobility. However, these activities were not derived from other DIFs such as DIF-2 and DIF-3. Identification of these DRFs found in this study would provide insight into the mechanism by which the development of the erkB- mutant is restored and how these factors act in the normal development of Dictyostelium. |
| Volume | 21(8) |
| Pages | 829-34 |
| Published | 2004-8-1 |
| DOI | 10.2108/zsj.21.829 |
| PII | JST.JSTAGE/zsj/21.829 |
| PMID | 15333995 |
| MeSH | Animals Cell Differentiation / physiology* Chromatography, High Pressure Liquid Dictyostelium / metabolism* Gene Expression Regulation, Developmental* Hexanones / metabolism* Mitogen-Activated Protein Kinase 1 / metabolism* Morphogenesis Pentanones / metabolism Signal Transduction / physiology* |
| IF | 0.843 |
| Times Cited | 3 |
| WOS Category | ZOOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | S00409 |