RRC ID 29067
著者 Rivero F, Muramoto T, Meyer AK, Urushihara H, Uyeda TQ, Kitayama C.
タイトル A comparative sequence analysis reveals a common GBD/FH3-FH1-FH2-DAD architecture in formins from Dictyostelium, fungi and metazoa.
ジャーナル BMC Genomics
Abstract BACKGROUND:Formins are multidomain proteins defined by a conserved FH2 (formin homology 2) domain with actin nucleation activity preceded by a proline-rich FH1 (formin homology 1) domain. Formins act as profilin-modulated processive actin nucleators conserved throughout a wide range of eukaryotes.
RESULTS:We present a detailed sequence analysis of the 10 formins (ForA to J) identified in the genome of the social amoeba Dictyostelium discoideum. With the exception of ForI and ForC all other formins conform to the domain structure GBD/FH3-FH1-FH2-DAD, where DAD is the Diaphanous autoinhibition domain and GBD/FH3 is the Rho GTPase-binding domain/formin homology 3 domain that we propose to represent a single domain. ForC lacks a FH1 domain, ForI lacks recognizable GBD/FH3 and DAD domains and ForA, E and J have additional unique domains. To establish the relationship between formins of Dictyostelium and other organisms we constructed a phylogenetic tree based on the alignment of FH2 domains. Real-time PCR was used to study the expression pattern of formin genes. Expression of forC, D, I and J increased during transition to multi-cellular stages, while the rest of genes displayed less marked developmental variations. During sexual development, expression of forH and forI displayed a significant increase in fusion competent cells.
CONCLUSION:Our analysis allows some preliminary insight into the functionality of Dictyostelium formins: all isoforms might display actin nucleation activity and, with the exception of ForI, might also be susceptible to autoinhibition and to regulation by Rho GTPases. The architecture GBD/FH3-FH1-FH2-DAD appears common to almost all Dictyostelium, fungal and metazoan formins, for which we propose the denomination of conventional formins, and implies a common regulatory mechanism.
巻・号 6
ページ 28
公開日 2005-3-1
DOI 10.1186/1471-2164-6-28
PII 1471-2164-6-28
PMID 15740615
PMC PMC555941
MeSH Actin Cytoskeleton Actins / chemistry Amino Acid Sequence Animals Caenorhabditis elegans Carrier Proteins / chemistry DNA, Complementary / metabolism Databases, Protein Dictyostelium / metabolism* Drosophila Fungal Proteins / chemistry* Gene Expression Regulation Humans Microfilament Proteins / chemistry Molecular Sequence Data Phylogeny Protein Isoforms Protein Structure, Tertiary Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae / metabolism Schizosaccharomyces / metabolism Sequence Analysis, DNA / methods* Sequence Homology, Amino Acid
IF 3.594
引用数 56
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
細胞性粘菌 S00041 S00044