RRC ID 29103
Author Chen ZH, Schaap P.
Title The prokaryote messenger c-di-GMP triggers stalk cell differentiation in Dictyostelium.
Journal Nature
Abstract Cyclic di-(3′:5′)-guanosine monophosphate (c-di-GMP) is a major prokaryote signalling intermediate that is synthesized by diguanylate cyclases and triggers sessility and biofilm formation. We detected the first eukaryote diguanylate cyclases in all major groups of Dictyostelia. On food depletion, Dictyostelium discoideum amoebas collect into aggregates, which first transform into migrating slugs and then into sessile fruiting structures. These structures consist of a spherical spore mass that is supported by a column of stalk cells and a basal disk. A polyketide, DIF-1, which induces stalk-like cells in vitro, was isolated earlier. However, its role in vivo proved recently to be restricted to basal disk formation. Here we show that the Dictyostelium diguanylate cyclase, DgcA, produces c-di-GMP as the morphogen responsible for stalk cell differentiation. Dictyostelium discoideum DgcA synthesized c-di-GMP in a GTP-dependent manner and was expressed at the slug tip, which is the site of stalk cell differentiation. Disruption of the DgcA gene blocked the transition from slug migration to fructification and the expression of stalk genes. Fructification and stalk formation were restored by exposing DgcA-null slugs to wild-type secretion products or to c-di-GMP. Moreover, c-di-GMP, but not cyclic di-(3′:5′)-adenosine monophosphate, induced stalk gene expression in dilute cell monolayers. Apart from identifying the long-elusive stalk-inducing morphogen, our work also identifies a role for c-di-GMP in eukaryotes.
Volume 488(7413)
Pages 680-3
Published 2012-8-30
DOI 10.1038/nature11313
PII nature11313
PMID 22864416
PMC PMC3939355
MeSH Biological Assay Cell Differentiation* Cyclic GMP / analogs & derivatives* Cyclic GMP / metabolism Dictyostelium / cytology* Dictyostelium / genetics Dictyostelium / growth & development Dictyostelium / metabolism* Fruiting Bodies, Fungal / metabolism Guanosine Triphosphate / metabolism Molecular Sequence Data Prokaryotic Cells / metabolism* Protozoan Proteins / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Second Messenger Systems*
IF 43.07
Times Cited 61
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Cellular slime molds S90402 S90403