RRC ID 29134
Author Nakagawa Y, Oda K, Nakada S.
Title The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.
Journal J Virol
Abstract We indicated that the PB1 and PA subunits of RNA polymerase and nucleoprotein (NP) can support replication of the influenza virus genome as well as transcription to yield uncapped poly(A)(+)-RNA (Y. Nakagawa, N. Kimura, T. Toyoda, K. Mizumoto, A. Ishihama, K. Oda, and S. Nakada, J. Virol. 69:728-733, 1995). To analyze the functions of the PB1 and PA subunits in replication and transcription, YP1N clones in which the PB1 and NP genes can be expressed in response to dexamethasone were established. cRNA was transcribed from model viral RNA (vRNA), but vRNA synthesis from model cRNA was not detected in YP1N clones. Furthermore, poly(A)(+)-RNA directed from model vRNA was synthesized in YP1N clones. These results indicated that PB1 and NP can support the syntheses of cRNA and poly(A)(+)-RNA and that the PA subunit, in addition to that of PB1 and to NP, is required for vRNA synthesis. In summary, the PB1 subunit is involved in the catalytic activities of nucleotide elongation, and the PA subunit may act as an allosteric modulator and cause a conformational change from a cRNA-to a vRNA-synthesizing form of the PB1 subunit.
Volume 70(9)
Pages 6390-4
Published 1996-9-1
DOI 10.1128/JVI.70.9.6390-6394.1996
PMID 8709268
PMC PMC190666
MeSH Animals Base Sequence Cell Line Clone Cells DNA Primers DNA-Directed RNA Polymerases / metabolism* Dexamethasone / pharmacology Gene Expression / drug effects Genes, Viral Genome, Viral* Mice Molecular Sequence Data Nucleocapsid Proteins Nucleoproteins* Orthomyxoviridae / genetics Orthomyxoviridae / physiology* Polymerase Chain Reaction RNA, Complementary / biosynthesis* RNA, Viral / biosynthesis* Transcription, Genetic Viral Core Proteins / metabolism* Viral Proteins / biosynthesis Viral Proteins / metabolism* Virus Replication*
IF 4.501
Times Cited 52
DNA material pT7/NS-CATc (RDB02055)