RRC ID 29162
Author Miyake T, Kanayama M, Sammoto H, Ono B.
Title A novel cis-acting cysteine-responsive regulatory element of the gene for the high-affinity glutathione transporter of Saccharomyces cerevisiae.
Journal Mol Genet Genomics
Abstract We cloned a DNA fragment from Saccharomyces cerevisiae that complemented the deficiency in high-affinity glutathione transport activity conferred by a gsh11 mutation, and found that the ORF responsible was YJL212c, which had already been designated as OPT1 and HGT1 by others. Northern analysis clearly demonstrated that this ORF, now referred to as OPT1/ HGT1/ GSH11, was induced by sulfur starvation and repressed by adding cysteine to the growth medium. Reporter gene assays showed that a segment spanning the region between positions -371 and -355 was essential for the regulation of this gene. A sequence of 9 nt, CCGCCACAC (from -364 to -356), in this region was shown to be required for protein binding, using an electrophoretic mobility shift assay. Based on these results, we propose that CCGCCACAC comprises the core of a cis-acting element involved in cysteine-responsive gene regulation in S. cerevisiae.
Volume 266(6)
Pages 1004-11
Published 2002-2-1
DOI 10.1007/s00438-001-0625-6
PMID 11862495
MeSH Base Sequence Binding Sites / genetics Blotting, Northern Carrier Proteins / genetics* Cloning, Molecular Cysteine / metabolism Cysteine / pharmacology* DNA, Fungal / genetics Gene Expression Regulation, Fungal / drug effects Glutathione / metabolism Glutathione / pharmacology Membrane Transport Proteins RNA, Messenger / genetics RNA, Messenger / metabolism Regulatory Sequences, Nucleic Acid / genetics* Saccharomyces cerevisiae / drug effects* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / growth & development
IF 2.797
Times Cited 13
DNA material HGT1/GSH11 (RDB02979)