| RRC ID |
29170
|
| Author |
Hanano S, Sugita M, Sugiura M.
|
| Title |
Structure and expression of the tobacco nuclear gene encoding RNA-binding protein RZ-1: the existence of an intron in the 3'-untranslated region.
|
| Journal |
DNA Res
|
| Abstract |
We have previously characterized a tobacco cDNA encoding a novel type RNA-binding protein (RZ-1), which contains a zinc finger motif in addition to a consensus sequence-type RNA-binding domain and is localized in the nucleus. Here we isolated its genomic clone from a Nicotiana sylvestris genomic library. Southern blot analysis suggested that RZ-1 is coded for by a single locus per haploid genome. Comparison of the cDNA and genomic sequences indicated that the RZ-1 gene contains two introns, one in the coding region and another in the 3'-untranslated region. RT-PCR and ribonuclease protection analyses showed that splicing of RZ-1 pre-mRNA occurs efficiently. The RZ-1 protein is actively synthesized in rapidly dividing tobacco cells, as demonstrated by immunoblot analysis.
|
| Volume |
3(2)
|
| Pages |
65-71
|
| Published |
1996-4-30
|
| DOI |
10.1093/dnares/3.2.65
|
| PMID |
8804857
|
| MeSH |
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Gene Expression Regulation, Plant
Introns
Molecular Sequence Data
Nuclear Proteins / chemistry
Nuclear Proteins / genetics
Nuclear Proteins / isolation & purification
Plant Proteins / chemistry*
Plant Proteins / genetics*
Plants, Toxic*
Protein Biosynthesis*
RNA Splicing
RNA-Binding Proteins / chemistry*
RNA-Binding Proteins / genetics*
Sequence Analysis, DNA
Tobacco / chemistry
Tobacco / genetics*
Transcription, Genetic
|
| IF |
4.009
|
| Resource |
| DNA material |
pGen N54 (RDB01817)
pgRZ-1 ES (RDB01818) |
