RRC ID 29175
Author Hanano S, Sugita M, Sugiura M.
Title Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells.
Journal Plant Mol Biol
Abstract A cDNA encoding a protein with a consensus sequence-type RNA-binding domain (CS-RBD) has been isolated from a Nicotiana sylvestris cDNA library. The deduced protein (designated 'RZ-1') contains CS-RBD in its N-terminal half, arginine/aspartic acid repeats in its center and a glycine-rich-C-terminal region in which a zinc finger motif of the CCHC type is present. The corresponding gene appears to be expressed constitutively in all tobacco organs. Immunocytochemical assays revealed that RZ-1 is localized in the nucleoplasm of tobacco cultured cells. Glycerol gradient fractionation of tobacco nuclear lysates showed that RZ-1 is associated with a large ribonucleoprotein particle of around 60 S in size. Nucleic acid-binding assays indicated that RZ-1 binds preferentially to poly (G) and both the CS-RBD and glycine-rich region are necessary for its binding activity. A possible role of RZ-1 is discussed.
Volume 31(1)
Pages 57-68
Published 1996-4-1
DOI 10.1007/BF00020606
PMID 8704159
MeSH Amino Acid Sequence Base Sequence Cell Nucleus / metabolism* DNA, Complementary Glycerol Molecular Sequence Data Plant Proteins / genetics* Plant Proteins / isolation & purification Plant Proteins / metabolism Plants, Toxic* Poly G / metabolism Protein Binding RNA-Binding Proteins / genetics* RNA-Binding Proteins / isolation & purification RNA-Binding Proteins / metabolism Ribonucleoproteins / metabolism* Tobacco / cytology Tobacco / metabolism*
IF 3.302
Times Cited 33
DNA material pNS RZ-1 (RDB01819)