論文 - 詳細
RRC ID | 29175 |
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著者 | Hanano S, Sugita M, Sugiura M. |
タイトル | Isolation of a novel RNA-binding protein and its association with a large ribonucleoprotein particle present in the nucleoplasm of tobacco cells. |
ジャーナル | Plant Mol Biol |
Abstract |
A cDNA encoding a protein with a consensus sequence-type RNA-binding domain (CS-RBD) has been isolated from a Nicotiana sylvestris cDNA library. The deduced protein (designated 'RZ-1') contains CS-RBD in its N-terminal half, arginine/aspartic acid repeats in its center and a glycine-rich-C-terminal region in which a zinc finger motif of the CCHC type is present. The corresponding gene appears to be expressed constitutively in all tobacco organs. Immunocytochemical assays revealed that RZ-1 is localized in the nucleoplasm of tobacco cultured cells. Glycerol gradient fractionation of tobacco nuclear lysates showed that RZ-1 is associated with a large ribonucleoprotein particle of around 60 S in size. Nucleic acid-binding assays indicated that RZ-1 binds preferentially to poly (G) and both the CS-RBD and glycine-rich region are necessary for its binding activity. A possible role of RZ-1 is discussed. |
巻・号 | 31(1) |
ページ | 57-68 |
公開日 | 1996-4-1 |
DOI | 10.1007/BF00020606 |
PMID | 8704159 |
MeSH | Amino Acid Sequence Base Sequence Cell Nucleus / metabolism* DNA, Complementary Glycerol Molecular Sequence Data Plant Proteins / genetics* Plant Proteins / isolation & purification Plant Proteins / metabolism Plants, Toxic* Poly G / metabolism Protein Binding RNA-Binding Proteins / genetics* RNA-Binding Proteins / isolation & purification RNA-Binding Proteins / metabolism Ribonucleoproteins / metabolism* Tobacco / cytology Tobacco / metabolism* |
IF | 3.302 |
引用数 | 33 |
WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
遺伝子材料 | pNS RZ-1 (RDB01819) |