論文 - 詳細
| RRC ID | 29180 |
|---|---|
| 著者 | Tsuchiya T, Tojo A, Shibuya M. |
| タイトル | Multi-copy introduction and high-level expression of interleukin-3 genes by retroviral vector superinfection. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We constructed a retroviral expression vector carrying multiple cloning sites. This vector was found to express efficiently the cloned gene. Using this vector and a helper virus-free system, a murine interleukin-3 (mlL-3) high-producing cell line was established by multiple cycles of infection with recombinant retroviruses carrying mlL-3 cDNA. The infected cells produced a considerable amount of mlL-3 and the concentration of mlL-3 in culture media increased as a function of the frequency of infection. High levels of mlL-3 cDNA, mRNA and protein in this cell line were confirmed by Southern, Northern and biological assays, respectively. These results suggest that artificial gene amplification is possible in a helper-free retroviral system. This should be applicable to efficient expression of bioactive molecules in a wide variety of mammalian cells including suspension cells. |
| 巻・号 | 158(2) |
| ページ | 576-83 |
| 公開日 | 1989-1-31 |
| DOI | 10.1016/s0006-291x(89)80088-9 |
| PII | S0006-291X(89)80088-9 |
| PMID | 2783848 |
| MeSH | Animals Blotting, Northern Blotting, Southern Genetic Engineering Genetic Vectors* Interleukin-3 / genetics* Mice Recombinant Proteins Retroviridae / genetics Superinfection |
| IF | 2.985 |
| 引用数 | 4 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pMNSM (RDB03043) |