RRC ID 29200
Author Shibahara S, Müller R, Taguchi H, Yoshida T.
Title Cloning and expression of cDNA for rat heme oxygenase.
Journal Proc Natl Acad Sci U S A
Abstract Two cDNA clones for rat heme oxygenase have been isolated from a rat spleen cDNA library in lambda gt11 by immunological screening using a specific polyclonal antibody. One of these clones has an insert of 1530 nucleotides that contains the entire protein-coding region. To confirm that the isolated cDNA encodes heme oxygenase, we transfected monkey kidney cells (COS-7) with the cDNA carried in a simian virus 40 vector. The heme oxygenase was highly expressed in endoplasmic reticulum of transfected cells. The nucleotide sequence of the cloned cDNA was determined and the primary structure of heme oxygenase was deduced. Heme oxygenase is composed of 289 amino acids and has one hydrophobic segment at its carboxyl terminus, which is probably important for the insertion of heme oxygenase into endoplasmic reticulum. The cloned cDNA was used to analyze the induction of heme oxygenase in rat liver by treatment with CoCl2 or with hemin. RNA blot analysis showed that both CoCl2 and hemin increased the amount of hybridizable mRNA, suggesting that these substances may act at the transcriptional level to increase the amount of heme oxygenase.
Volume 82(23)
Pages 7865-9
Published 1985-12-1
DOI 10.1073/pnas.82.23.7865
PMID 3865203
PMC PMC390870
MeSH Amino Acid Sequence Animals Base Sequence Cell Line Chlorocebus aethiops Cloning, Molecular DNA / genetics Gene Expression Regulation Heme Oxygenase (Decyclizing) / genetics* Heme Oxygenase (Decyclizing) / immunology Mixed Function Oxygenases / genetics* Protein Conformation RNA, Messenger / genetics Rats Solubility
IF 9.412
Times Cited 450
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pRH01 (RDB01208)