Reference - Detail
| RRC ID | 29251 |
|---|---|
| Author | Morita T, Fukuoka T, Imura T, Kitamoto D. |
| Title | Accumulation of cellobiose lipids under nitrogen-limiting conditions by two ustilaginomycetous yeasts, Pseudozyma aphidis and Pseudozyma hubeiensis. |
| Journal | FEMS Yeast Res |
| Abstract |
Some basidiomycetous yeast strains extracellularly produce cellobiose lipids (CLs), glycolipid biosurfactants which have strong fungicidal activity. The representative CL producer Ustilago maydis produces CLs together with the other glycolipids, mannosylerythritol lipids (MELs); the preference of the two glycolipids is affected considerably by the nitrogen source. To develop new CL producers, 12 MEL producers were cultured under the nitrogen-limited conditions. Pseudozyma aphidis and Pseudozyma. hubeiensis were characterized as new CL producers. CL production was induced on three strains, P. aphidis, Pseudozyma graminicola, and P. hubeiensis under these conditions. The putative homologous genes of U. maydis cyp1, which encodes a P450 monooxygenase, essential for CL biosynthesis, were partially amplified from their genomic DNA. The nucleotide sequences of the gene fragments from P. hubeiensis and P. aphidis shared identities with U. maydis cyp1 of 99% and 78%, respectively. Furthermore, all of the deduced translation products are tightly clustered in the phylogenic tree of the monooxygenase. These results suggest that the genes involved with CL biosynthesis must be widely distributed in the basidiomycetous fungi as well as the MEL biosynthesis genes, and thus, the genus Pseudozyma has great potential as a biosurfactant producer. |
| Volume | 13(1) |
| Pages | 44-9 |
| Published | 2013-2-1 |
| DOI | 10.1111/1567-1364.12005 |
| PMID | 22985214 |
| MeSH | Base Sequence Cellobiose / chemistry Cellobiose / metabolism* Cytochrome P-450 Enzyme System / genetics DNA, Fungal / chemistry DNA, Fungal / genetics DNA, Ribosomal Spacer / chemistry DNA, Ribosomal Spacer / genetics Fungal Proteins / genetics Glycolipids / chemistry Glycolipids / metabolism* Lipid Metabolism Lipids / chemistry Mixed Function Oxygenases / genetics Molecular Sequence Data Nitrogen / deficiency* Nitrogen / metabolism Phylogeny Sequence Analysis, DNA Ustilaginales / genetics Ustilaginales / metabolism* |
| IF | 3.193 |
| Times Cited | 14 |
| WOS Category | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 3931 JCM 10317 JCM 10318 JCM 10323 JCM 10324 JCM 11752 |