RRC ID 29268
著者 Kumar PA, Bhumika V, Ritika C, Bhaskar YV, Priyashanth P, Aravind R, Bindu E, Srinivas TN.
タイトル Algoriphagus shivajiensis sp. nov., isolated from Cochin back water, India.
ジャーナル Syst Appl Microbiol
Abstract Novel orange-pigmented, Gram-negative, rod-shaped, non-motile bacteria, designated strains NIO-S3(T) and NIO-S4, were isolated from a water sample collected from Cochin back waters, Thanneermukkom and Arookutty, Kerala, India. Both strains were positive for oxidase and catalase activities, and hydrolyzed gelatin and Tween 40. The predominant fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0 3OH, C16:1ω7c/C16:1ω6c (summed feature 3) and iso-C17:1ω9c/C16:0 10-methyl (summed feature 9), whereas MK-7 was the major respiratory quinone, and phosphatidylethanolamine, two unidentified phospholipids and one unidentified lipid were the only polar lipids. The DNA G+C content of the two strains was 43.7 and 43.6mol%, respectively. The 16S rRNA gene sequence analysis indicated that they were members of the genus Algoriphagus and closely related to Algoriphagus olei CC-Hsuan-617(T), Algoriphagus aquatilis A8-7(T), Algoriphagus aquaeductus LMG 24398(T) and Algoriphagus mannitolivorans DSM 15301(T), with pairwise sequence similarities of 96.8, 96.6, 96.2 and 96.2%, respectively. DNA-DNA hybridization between strains NIO-S3(T) and NIO-S4 showed a relatedness of 89%. Based on data from the current polyphasic study, the strains are proposed as a novel species of the genus Algoriphagus, for which the name Algoriphagus shivajiensis sp. nov. is proposed. The type strain of A. shivajiensis is NIO-S3(T) (=JCM 17885(T)=MTCC 11066(T)).
巻・号 36(2)
ページ 106-11
公開日 2013-3-1
DOI 10.1016/j.syapm.2012.11.006
PII S0723-2020(12)00161-0
PMID 23332681
MeSH Bacterial Typing Techniques Bacteroidetes / classification* Bacteroidetes / genetics Bacteroidetes / isolation & purification* Bacteroidetes / physiology Base Composition Catalase / metabolism Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Gelatin / metabolism Hydrolysis India Molecular Sequence Data Oxidoreductases / metabolism Phospholipids / metabolism Phylogeny Pigments, Biological / metabolism Polysorbates / metabolism Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Water Microbiology*
IF 3.224
引用数 4
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 17885