| RRC ID |
29269
|
| 著者 |
Shinozaki Y, Kikkawa Y, Sato S, Fukuoka T, Watanabe T, Yoshida S, Nakajima-Kambe T, Kitamoto HK.
|
| タイトル |
Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic force microscopy study.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica JCM10317 (PaE) was analyzed by surface plasmon resonance (SPR). The adsorption of PaE and the degradation rate for polyester films were quantitatively monitored by a positive and negative SPR signal shifts, respectively. The decrease in SPR signal and the erosion depth of amorphous poly(L-lactide) (a-PLLA) film measured by atomic force microscopy (AFM) had a linear relationship, and the weight loss was estimated from the AFM data combined with a density of a-PLLA film. Furthermore, SPR sensorgrams for various polyester films showed that degradation rate of poly(ε-caprolactone) and poly(butylene succinate-co-adipate) which contain C6 units was higher than that of other polyesters such as poly(butylene succinate) and a-PLLA. These results suggest that C6 is the preferred chain length as substrates for PaE.
|
| 巻・号 |
97(19)
|
| ページ |
8591-8
|
| 公開日 |
2013-10-1
|
| DOI |
10.1007/s00253-012-4673-0
|
| PMID |
23339012
|
| MeSH |
Carboxylic Ester Hydrolases / metabolism*
Microscopy, Atomic Force
Polyesters / metabolism*
Substrate Specificity
Surface Plasmon Resonance
Ustilaginales / enzymology*
|
| IF |
3.53
|
| 引用数 |
18
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 10317 |
