論文 - 詳細
| RRC ID | 29271 |
|---|---|
| 著者 | Gorlas A, Geslin C. |
| タイトル | A simple procedure to determine the infectivity and host range of viruses infecting anaerobic and hyperthermophilic microorganisms. |
| ジャーナル | Extremophiles |
| Abstract |
Plaque assay is the method traditionally used to isolate and purify lytic viruses, to determine the viral titer and host range. Whereas most bacterioviruses are either temperate or lytic, the majority of known archeoviruses are not lytic (i.e. they are temperate or chronic). In view of the widespread occurrence of such viruses in extreme environments, we designed an original method, called the inverted spot test, to determine the host range and infectivity of viruses isolated from anaerobic hyperthermophilic and sulfur-reducing microorganisms. Here, we used this approach to prove for the first time the infectivity of Pyrococcus abyssi virus 1 (PAV1) and to confirm the host range of Thermococcus prieurii virus 1 (TPV1), the only two viruses isolated so far from any of the described marine hyperthermophilic archaea (Euryarchaeota phylum, Thermococcales order). |
| 巻・号 | 17(2) |
| ページ | 349-55 |
| 公開日 | 2013-3-1 |
| DOI | 10.1007/s00792-013-0513-0 |
| PMID | 23340763 |
| MeSH | Archaeal Viruses / isolation & purification Archaeal Viruses / pathogenicity* Host Specificity Pyrococcus / classification Pyrococcus / virology* Thermococcus / classification Thermococcus / virology* Virology / methods |
| IF | 2.462 |
| 引用数 | 3 |
| WOS 分野 | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 8422 JCM 8558 JCM 9974 JCM 11827 JCM 12380 JCM 13517 JCM 16307 |