| RRC ID |
29276
|
| 著者 |
Nishimura H, Azami Y, Miyagawa M, Hashimoto C, Yoshimura T, Hemmi H.
|
| タイトル |
Biochemical evidence supporting the presence of the classical mevalonate pathway in the thermoacidophilic archaeon Sulfolobus solfataricus.
|
| ジャーナル |
J Biochem
|
| Abstract |
The existence of the classical mevalonate (MVA) pathway was examined in the thermoacidophilic archaeon Sulfolobus solfataricus. The pathway is considered uncommon among archaea because the genes of the orthologues of phosphomevalonate kinase (PMK) and/or diphosphomevalonate decarboxylase (DMD) are absent in the genomes of most archaea. Instead, the modified MVA pathway, which involves isopentenyl phosphate kinase (IPK), has been proposed to exist in the archaea that lack the classical pathway. However, some archaea including S. solfataricus possess the genes of the orthologues of both IPK and all enzymes of the classical pathway. Biochemical characterization using recombinant proteins showed that the orthologues of the enzymes catalyzing the late steps of the classical pathway, i.e. MVA kinase, PMK and DMD, are all active. Moreover, in vitro conversion of the intermediates in the classical and modified pathways by cell-free extract from S. solfataricus indicated that only the classical pathway likely works in the organism.
|
| 巻・号 |
153(5)
|
| ページ |
415-20
|
| 公開日 |
2013-5-1
|
| DOI |
10.1093/jb/mvt006
|
| PII |
mvt006
|
| PMID |
23378249
|
| MeSH |
Mevalonic Acid / metabolism*
Models, Biological
Phosphotransferases (Phosphate Group Acceptor) / metabolism
Protein Kinases / metabolism
Signal Transduction / physiology
Sulfolobus solfataricus / metabolism*
Terpenes / metabolism
|
| IF |
2.476
|
| 引用数 |
21
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 11322 |
