論文 - 詳細
| RRC ID | 29281 |
|---|---|
| 著者 | Song JY, Seo YB, Hong SK, Chang YK. |
| タイトル | Heterologous expression of a putative K+/H+ antiporter of S. coelicolor A3(2) enhances K+, acidic-pH shock tolerances, and geldanamycin secretion. |
| ジャーナル | J Microbiol Biotechnol |
| Abstract |
Heterologous expression of a putative K+/H+ antiporter of Streptomyces coelicolor A3(2) (designated as sha4) in E. coli and Streptomyces hygroscopicus JCM4427 showed enhanced tolerance to K+ stress, acidic-pH shock, and/or geldanamycin production under K+ stress. In a series of K+ extrusion experiments with sha4-carrying E. coli deficient in the K+/H+ antiporter, a restoration of impaired K+ extrusion activity was observed. Based on this, it was concluded that sha4 was a true K+/H+ antiporter. In different sets of experiments, the sha4-carrying E. coli showed significantly improved tolerances to K+ stresses and acidic-pH shock, whereas sha4-carrying S. hygroscopicus showed an improvement in K+ stress tolerance only. The sha4-carrying S. hygroscopicus showed much higher geldanamycin productivity than the control under K+ stress condition. In another set of experiments with a production medium, the secretion of geldanamycin was also significantly enhanced by the expression of sha4. |
| 巻・号 | 23(2) |
| ページ | 149-55 |
| 公開日 | 2013-2-1 |
| DOI | 10.4014/jmb.1207.07030 |
| PII | JMB023-02-03 |
| PMID | 23412055 |
| MeSH | Acids / toxicity* Anti-Bacterial Agents / metabolism* Benzoquinones / metabolism* Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Genetic Complementation Test Lactams, Macrocyclic / metabolism* Potassium / toxicity* Potassium-Hydrogen Antiporters / genetics Potassium-Hydrogen Antiporters / metabolism* Streptomyces coelicolor / enzymology* Streptomyces coelicolor / genetics Stress, Physiological* |
| IF | 1.992 |
| 引用数 | 2 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 4427 |