RRC ID 29282
Author Koyama S, Konishi MA, Ohta Y, Miwa T, Hatada Y, Toyofuku T, Maruyama T, Nogi Y, Kato C, Tsubouchi T.
Title Attachment and detachment of living microorganisms using a potential-controlled electrode.
Journal Mar Biotechnol (NY)
Abstract We developed an electrical modulation method for attachment and detachment of microorganisms. Living microorganisms suspended in non-nutritive media such as PBS⁻ and artificial seawater were attracted by and selectively attached to indium tin oxide (ITO)/glass electrode regions to which a negative potential was applied. The microorganisms suspended in LB medium and glucose solution were not attracted to the ITO electrode. Dead microorganisms were not attracted to the ITO electrode. The living microorganisms were retrieved after detachment from the ITO electrode by application of a high-frequency triangular wave potential. When we applied this method to separate microorganisms from deep-sea sediment, bacteria belonging to 19 phyla and 23 classes were collected without undesirable high molecular weight contaminants such as humic acids. At the phylum and class level, respectively, 95 and 87 % of the phylotypes among electrically retrieved bacteria were common to the gene clones from the direct sediment DNA extraction. This technique is a novel useful method to prepare bacterial cells in a single population or a community for metagenomic analyses.
Volume 15(4)
Pages 461-75
Published 2013-8-1
DOI 10.1007/s10126-013-9495-2
PMID 23420537
PMC PMC3695320
MeSH Bacteria / genetics Bacteria / metabolism* Bacteria / ultrastructure Base Sequence Computational Biology DNA Primers / genetics Electrodes / microbiology* Electromagnetic Fields Geologic Sediments / microbiology* Japan Metagenomics / methods* Microscopy, Atomic Force Microscopy, Electron, Scanning Molecular Sequence Data Phylogeny Seawater / microbiology* Sequence Analysis, DNA Soil Microbiology* Species Specificity Tin Compounds / metabolism*
IF 2.798
Times Cited 8
General Microbes JCM 1465 JCM 1649