論文 - 詳細
| RRC ID | 29291 |
|---|---|
| 著者 | Matsumoto A, Shimizu Y, Takemoto C, Ueda T, Uchiumi T, Ito K. |
| タイトル | Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8. |
| ジャーナル | Acta Crystallogr Sect F Struct Biol Cryst Commun |
| Abstract |
Peptidyl-tRNA is produced from the ribosome as a result of aborted translation. Peptidyl-tRNA hydrolase cleaves the ester bond between the peptide and the tRNA of peptidyl-tRNA molecules, to recycle tRNA for further rounds of protein synthesis. In this study, peptidyl-tRNA hydrolase from Thermus thermophilus HB8 (TthPth) was crystallized using 2-methyl-2,4-pentanediol as a precipitant. The crystals belonged to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a=47.45, b=53.92, c=58.67 Å, and diffracted X-rays to atomic resolution (beyond 1.0 Å resolution). The asymmetric unit is expected to contain one TthPth molecule, with a solvent content of 27.13% (VM=1.69 Å3 Da(-1)). The structure is being solved by molecular replacement. |
| 巻・号 | 69(Pt 3) |
| ページ | 332-5 |
| 公開日 | 2013-3-1 |
| DOI | 10.1107/S1744309113003424 |
| PII | S1744309113003424 |
| PMID | 23519816 |
| PMC | PMC3606586 |
| MeSH | Amino Acid Sequence Bacterial Proteins / chemistry* Bacterial Proteins / genetics Carboxylic Ester Hydrolases / chemistry* Carboxylic Ester Hydrolases / genetics Crystallization Crystallography, X-Ray Escherichia coli / enzymology Escherichia coli / genetics Glycols / chemistry Molecular Sequence Data Recombinant Proteins / chemistry Recombinant Proteins / genetics Thermus thermophilus / chemistry* Thermus thermophilus / enzymology Thermus thermophilus / genetics |
| IF | 0.968 |
| 引用数 | 1 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS CRYSTALLOGRAPHY |
| リソース情報 | |
| 遺伝子材料 | Thermus thermophilus expression plasmid TEx17E12 (THR006908) |
| 一般微生物 | JCM 10941 |