論文 - 詳細
| RRC ID | 29307 |
|---|---|
| 著者 | Sawa N, Koga S, Okamura K, Ishibashi N, Zendo T, Sonomoto K. |
| タイトル | Identification and characterization of novel multiple bacteriocins produced by Lactobacillus sakei D98. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIM:To characterize novel multiple bacteriocins produced by Lactobacillus sakei D98. METHODS AND RESULTS:Lactobacillus sakei D98 isolated from Shubo (rice malt) produced at least three bacteriocins. Using three purification steps, three novel antimicrobial peptides termed sakacin D98a, sakacin D98b and sakacin D98c were purified from the culture supernatant. Amino acid and DNA sequencing analysis revealed that the sakacins D98a, D98b and D98c are novel class IIa-like or class IId bacteriocins. In particular, sakacin D98b has a variant pediocin-box sequence, YANGVXC (with Ala instead of Gly), and a different location for the disulfide bridge (Cys(11) and Cys(18)) from that found in other class IIa bacteriocins. CONCLUSIONS:Three novel bacteriocins were identified from Lactobacillus sakei D98. Their antimicrobial spectra and intensities indicate that these sakacins would have different modes of action. In addition, sakacin D98b showed low inhibitory activity against Listeria, probably due to the differences in amino acids and position of the disulfide bridge compared with the other class IIa bacteriocins. SIGNIFICANCE AND IMPACT OF STUDY:Sakacins D98a and D98c are novel bacteriocins belonging to class IId bacteriocins. On the other hand, sakacin D98b, a class IIa-like bacteriocin, has a unique internal structure and activity spectrum. |
| 巻・号 | 115(1) |
| ページ | 61-9 |
| 公開日 | 2013-7-1 |
| DOI | 10.1111/jam.12226 |
| PMID | 23594273 |
| MeSH | Amino Acid Sequence Anti-Bacterial Agents / biosynthesis Anti-Bacterial Agents / chemistry* Anti-Bacterial Agents / pharmacology* Bacteriocins / biosynthesis Bacteriocins / chemistry* Bacteriocins / pharmacology* Base Sequence Disulfides / chemistry Lactobacillus / metabolism* Molecular Sequence Data Sequence Analysis, DNA |
| IF | 3.066 |
| 引用数 | 18 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1157 JCM 2257 JCM 2504 JCM 5803 JCM 5885 JCM 6124 |