RRC ID 29308
著者 Nakajima T, Ara W, Kagawa S, Moore JE, Matsubara K, Matsuda M.
タイトル Absence of intervening sequences and point mutations in the V domain within 23S rRNA in Campylobacter lari isolates.
ジャーナル Folia Microbiol (Praha)
Abstract Although the absence of intervening sequences (IVSs) within the 23S rRNA genes in Campylobacter lari isolates has been described, there are apparently no reports regarding correlations between the nucleotide sequences of 23S rRNA genes and erythromycin (Ery) susceptibility in C. lari isolates. Here, we determined the minimum inhibitory concentrations of 35 C. lari isolates [n = 19 for urease-positive thermophilic Campylobacter (UPTC); n = 16 urease-negative (UN) C. lari] obtained from Asia, Europe, and North America. We found that the 18 isolates were resistant to the Ery (defined as ≧8 μg/mL), and three isolates, UPTC A1, UPTC 92251, and UPTC 504, showed increased resistance (16 μg/mL). No correlations between the IVSs in the helix 45 region within the 23S rRNA gene sequences and Ery resistance were identified in the C. lari isolates examined. In addition, no point mutations occurred at any expected or putative position within the V domain in the isolates. In conclusion, antibiotic resistance against the macrolide erythromycin is mediated through an alternative pathway to that described above.
巻・号 58(6)
ページ 607-13
公開日 2013-11-1
DOI 10.1007/s12223-013-0250-x
PMID 23595231
MeSH Animals Anti-Bacterial Agents / pharmacology Asia Base Sequence Campylobacter Infections / microbiology Campylobacter Infections / veterinary Campylobacter lari / drug effects Campylobacter lari / genetics* Campylobacter lari / isolation & purification Drug Resistance, Bacterial Environmental Microbiology Erythromycin / pharmacology Europe Humans Introns* Microbial Sensitivity Tests Molecular Sequence Data North America Point Mutation* RNA, Ribosomal, 23S / genetics* Sequence Analysis, DNA
IF 1.73
引用数 0
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM2530