論文 - 詳細
| RRC ID | 29327 |
|---|---|
| 著者 | Ren P, Xu L, Yang Y, He S, Liu W, Ringø E, Zhou Z. |
| タイトル | Lactobacillus planarum subsp. plantarum JCM 1149 vs. Aeromonas hydrophila NJ-1 in the anterior intestine and posterior intestine of hybrid tilapia Oreochromis niloticus ♀ × Oreochromis aureus ♂: an ex vivo study. |
| ジャーナル | Fish Shellfish Immunol |
| Abstract |
To investigate the ex vivo interactions of probiotic-pathogen-host in warm-water fish, hybrid tilapia (Oreochromis niloticus♀ × Oreochromis aureus♂) were sacrificed to isolate anterior and posterior intestine for incubation with phosphate-buffered saline (PBS; pH 7.2) as the control, Lactobacillus plantarum JCM 1149 at 1.0 × 10(9) CFU/ml, Aeromonas hydrophila NJ-1 at 1.0 × 10(8) CFU/ml, or the both combination. Denaturing Gradient Gel Electrophoresis (DGGE) fingerprint and consequent sequence analysis confirmed anterior intestine sac was more prone to the colonization of L. plantarum JCM 1149 and A. hydrophila NJ-1 than the posterior part. L. plantarum JCM 1149 and A. hydrophila NJ-1 inhibited the population each other in anterior or posterior sac, indicating their competition for the colonization. The induced expression of HSP70, IL-1β and TNF-α in the anterior sac by the addition of L. plantarum JCM 1149 or A. hydrophila NJ-1 demonstrated the activity and a local immune response of ex vivo anterior sac. Compared with posterior intestine, higher population colonization and more sensitive immune response of anterior sac indicated differential patterns for the probiotic-pathogen-host interactions. Scanning electronic microscopy (SEM) observation showed that pathogen A. hydrophila NJ-1 damaged the anterior intestine, which was alleviated by the pretreatment of L. plantarum JCM 1149, showing its probiotic effect. |
| 巻・号 | 35(1) |
| ページ | 146-53 |
| 公開日 | 2013-7-1 |
| DOI | 10.1016/j.fsi.2013.04.023 |
| PII | S1050-4648(13)00525-1 |
| PMID | 23664914 |
| MeSH | Aeromonas hydrophila / physiology* Animal Feed / analysis Animals Denaturing Gradient Gel Electrophoresis / veterinary Hybridization, Genetic Intestines / microbiology* Lactobacillus plantarum / physiology* Microscopy, Electron, Scanning / veterinary Probiotics / pharmacology* Real-Time Polymerase Chain Reaction / veterinary Tilapia / genetics Tilapia / microbiology* |
| IF | 3.298 |
| 引用数 | 15 |
| WOS 分野 | VETERINARY SCIENCES FISHERIES MARINE & FRESHWATER BIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1149 |