RRC ID 29327
Author Ren P, Xu L, Yang Y, He S, Liu W, Ringø E, Zhou Z.
Title Lactobacillus planarum subsp. plantarum JCM 1149 vs. Aeromonas hydrophila NJ-1 in the anterior intestine and posterior intestine of hybrid tilapia Oreochromis niloticus ♀ × Oreochromis aureus ♂: an ex vivo study.
Journal Fish Shellfish Immunol
Abstract To investigate the ex vivo interactions of probiotic-pathogen-host in warm-water fish, hybrid tilapia (Oreochromis niloticus♀ × Oreochromis aureus♂) were sacrificed to isolate anterior and posterior intestine for incubation with phosphate-buffered saline (PBS; pH 7.2) as the control, Lactobacillus plantarum JCM 1149 at 1.0 × 10(9) CFU/ml, Aeromonas hydrophila NJ-1 at 1.0 × 10(8) CFU/ml, or the both combination. Denaturing Gradient Gel Electrophoresis (DGGE) fingerprint and consequent sequence analysis confirmed anterior intestine sac was more prone to the colonization of L. plantarum JCM 1149 and A. hydrophila NJ-1 than the posterior part. L. plantarum JCM 1149 and A. hydrophila NJ-1 inhibited the population each other in anterior or posterior sac, indicating their competition for the colonization. The induced expression of HSP70, IL-1β and TNF-α in the anterior sac by the addition of L. plantarum JCM 1149 or A. hydrophila NJ-1 demonstrated the activity and a local immune response of ex vivo anterior sac. Compared with posterior intestine, higher population colonization and more sensitive immune response of anterior sac indicated differential patterns for the probiotic-pathogen-host interactions. Scanning electronic microscopy (SEM) observation showed that pathogen A. hydrophila NJ-1 damaged the anterior intestine, which was alleviated by the pretreatment of L. plantarum JCM 1149, showing its probiotic effect.
Volume 35(1)
Pages 146-53
Published 2013-7-1
DOI 10.1016/j.fsi.2013.04.023
PII S1050-4648(13)00525-1
PMID 23664914
MeSH Aeromonas hydrophila / physiology* Animal Feed / analysis Animals Denaturing Gradient Gel Electrophoresis / veterinary Hybridization, Genetic Intestines / microbiology* Lactobacillus plantarum / physiology* Microscopy, Electron, Scanning / veterinary Probiotics / pharmacology* Real-Time Polymerase Chain Reaction / veterinary Tilapia / genetics Tilapia / microbiology*
IF 3.298
Times Cited 15
General Microbes JCM 1149