RRC ID 29332
Author Kitagawa W, Ozaki T, Nishioka T, Yasutake Y, Hata M, Nishiyama M, Kuzuyama T, Tamura T.
Title Cloning and heterologous expression of the aurachin RE biosynthesis gene cluster afford a new cytochrome P450 for quinoline N-hydroxylation.
Journal Chembiochem
Abstract Aurachin RE is a prenylated quinoline antibiotic that was first isolated from the genus Rhodococcus. It shows potent antibacterial activity against a variety of Gram-positive bacteria. Here we have identified a minimal biosynthesis gene cluster for aurachin RE in Rhodococcus erythropolis JCM 6824 by using random transposon mutagenesis and heterologous production. The Rhodococcus aurachin (rau) gene cluster consists of genes encoding cytochrome P450 (rauA), prenyltransferase, polyketide synthase, and farnesyl pyrophosphate synthase, as well as others including genes involved in regulation and transport. Markerless gene disruption of rauA resulted in the complete loss of aurachin RE production and in the accumulation of a new aurachin derivative lacking the N-hydroxy group. When the recombinant RauA was expressed in Escherichia coli, it catalyzed N-hydroxylation of the derivative to form aurachin RE. This study establishes the biosynthetic pathway of aurachin RE and provides experimental evidence for the role of P450 RauA in catalyzing N-hydroxylation of the quinoline ring, which is indispensable for the antibacterial activity of aurachin RE.
Volume 14(9)
Pages 1085-93
Published 2013-6-17
DOI 10.1002/cbic.201300167
PMID 23677853
MeSH Anti-Bacterial Agents / biosynthesis* Anti-Bacterial Agents / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cloning, Molecular Cytochrome P-450 Enzyme System / genetics Cytochrome P-450 Enzyme System / metabolism* Hydroxylamines / chemistry Hydroxylamines / metabolism* Hydroxylation Multigene Family Quinolines / chemistry Quinolines / metabolism* Quinolones / chemistry Quinolones / metabolism* Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Rhodococcus / genetics Rhodococcus / metabolism
IF 2.576
Times Cited 15
General Microbes JCM 3201 JCM 6824