論文 - 詳細
| RRC ID | 29347 |
|---|---|
| 著者 | Huang Y, Sun Y, Ma S, Chen L, Zhang H, Deng Y. |
| タイトル | Isolation and characterization of Keratinibaculum paraultunense gen. nov., sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
A novel thermophilic, anaerobic, keratinolytic bacterium designated KD-1 was isolated from grassy marshland. Strain KD-1 was a spore-forming rod with a Gram-positive type cell wall, but stained Gram-negative. The temperature, pH, and NaCl concentration range necessary for growth was 30-65 °C (optimum 55 °C), 6.0-10.5 (optimum 8.0-8.5), and 0-6% (optimum 0.2%) (w/v), respectively. Strain KD-1 possessed extracellular keratinase, and the optimum activity of the crude enzyme was pH 8.5 and 70 °C. The enzyme was identified as a thermostable serine-type protease. The strain was sensitive to rifampin, chloramphenicol, kanamycin, and tetracycline and was resistant to erythromycin, neomycin, penicillin, and streptomycin. The main cellular fatty acid was predominantly C15:0 iso (64%), and the G+C content was 28 mol%. Morphological and physiological characterization, together with phylogenetic analysis based on 16S rRNA gene sequencing identified KD-1 as a new species of a novel genus of Clostridiaceae with 95.3%, 93.8% 16S rRNA gene sequence similarity to Clostridium ultunense BS(T) (DSM 10521(T)) and Tepidimicrobium xylanilyticum PML14(T) (= JCM 15035(T)), respectively. We propose the name Keratinibaculum paraultunense gen. nov., sp. nov., with KD-1 (=JCM 18769(T) =DSM 26752(T)) as the type strain. |
| 巻・号 | 345(1) |
| ページ | 56-63 |
| 公開日 | 2013-8-1 |
| DOI | 10.1111/1574-6968.12184 |
| PMID | 23710623 |
| MeSH | Animals Bacteria, Anaerobic / classification Bacteria, Anaerobic / enzymology* Bacteria, Anaerobic / genetics Bacteria, Anaerobic / isolation & purification* Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism* Chickens Enzyme Stability Feathers / metabolism Gram-Positive Bacteria / classification Gram-Positive Bacteria / genetics Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / metabolism* Hot Temperature Keratins / metabolism* Molecular Sequence Data Peptide Hydrolases / chemistry Peptide Hydrolases / genetics Peptide Hydrolases / metabolism* Phylogeny Soil Microbiology* |
| IF | 1.987 |
| 引用数 | 12 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 18769 |