RRC ID 29351
著者 Yamamoto S, Fukuoka T, Imura T, Morita T, Yanagidani S, Kitamoto D, Kitagawa M.
タイトル Production of a novel mannosylerythritol lipid containing a hydroxy fatty acid from castor oil by Pseudozyma tsukubaensis.
ジャーナル J Oleo Sci
Abstract Mannosylerythritol lipids (MELs) are glycolipid biosurfactants produced by various yeasts belonging to the genus Pseudozyma, which exhibit excellent surface activities as well as versatile biochemical activities. A study on P. tsukubaensis NBRC1940 as a mono-acetylated MEL (MEL-B) producer revealed that the yeast accumulated a novel glycolipid from castor oil at a yield of 22 g/L. Its main chemical structure was identified as 1-O-β-(2'-O-alka(e)noyl-3'-O-hydroxyalka(e)noyl-6'-O-acetyl-D-mannopyranosyl)-D-erythritol designated as "new MEL-B." The new MEL-B, comprising a hydroxy fatty acid had a reduced surface tension of 28.5 mN/m at a critical micelle concentration (CMC) of 2.2×10⁻⁵ M in water. The observed CMC was 5-fold higher than that of conventional MEL-B. When conventional MEL-B was dispersed in water, it self-assembled to form the lamellar (L(α)) phase at a wide range of concentrations. In contrast, new MEL-B formed spherical oily droplets similar to the sponge (L₃) phase, which is observed in aqueous solutions of di-acetylated MEL (MEL-A). The data suggest that the newly identified MEL-B is likely to have a different structure and interfacial properties compared to the conventional MELs, and could facilitate an increase in the application of glycolipid biosurfactants.
巻・号 62(6)
ページ 381-9
公開日 2013-1-1
DOI 10.5650/jos.62.381
PII DN/JST.JSTAGE/jos/62.381
PMID 23728329
MeSH Basidiomycota / metabolism* Castor Oil / metabolism* Fatty Acids / chemistry* Glycolipids / biosynthesis* Glycolipids / chemistry* Micelles Surface Tension Surface-Active Agents* Water
IF 1.304
引用数 10
WOS 分野 FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED
リソース情報
一般微生物 JCM 10317