RRC ID |
29351
|
著者 |
Yamamoto S, Fukuoka T, Imura T, Morita T, Yanagidani S, Kitamoto D, Kitagawa M.
|
タイトル |
Production of a novel mannosylerythritol lipid containing a hydroxy fatty acid from castor oil by Pseudozyma tsukubaensis.
|
ジャーナル |
J Oleo Sci
|
Abstract |
Mannosylerythritol lipids (MELs) are glycolipid biosurfactants produced by various yeasts belonging to the genus Pseudozyma, which exhibit excellent surface activities as well as versatile biochemical activities. A study on P. tsukubaensis NBRC1940 as a mono-acetylated MEL (MEL-B) producer revealed that the yeast accumulated a novel glycolipid from castor oil at a yield of 22 g/L. Its main chemical structure was identified as 1-O-β-(2'-O-alka(e)noyl-3'-O-hydroxyalka(e)noyl-6'-O-acetyl-D-mannopyranosyl)-D-erythritol designated as "new MEL-B." The new MEL-B, comprising a hydroxy fatty acid had a reduced surface tension of 28.5 mN/m at a critical micelle concentration (CMC) of 2.2×10⁻⁵ M in water. The observed CMC was 5-fold higher than that of conventional MEL-B. When conventional MEL-B was dispersed in water, it self-assembled to form the lamellar (L(α)) phase at a wide range of concentrations. In contrast, new MEL-B formed spherical oily droplets similar to the sponge (L₃) phase, which is observed in aqueous solutions of di-acetylated MEL (MEL-A). The data suggest that the newly identified MEL-B is likely to have a different structure and interfacial properties compared to the conventional MELs, and could facilitate an increase in the application of glycolipid biosurfactants.
|
巻・号 |
62(6)
|
ページ |
381-9
|
公開日 |
2013-1-1
|
DOI |
10.5650/jos.62.381
|
PII |
DN/JST.JSTAGE/jos/62.381
|
PMID |
23728329
|
MeSH |
Basidiomycota / metabolism*
Castor Oil / metabolism*
Fatty Acids / chemistry*
Glycolipids / biosynthesis*
Glycolipids / chemistry*
Micelles
Surface Tension
Surface-Active Agents*
Water
|
IF |
1.304
|
引用数 |
10
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
|
リソース情報 |
一般微生物 |
JCM 10317 |