RRC ID 29410
著者 Yang G, Chen M, Zhou S, Liu Z, Yuan Y.
タイトル Sinorhodobacter ferrireducens gen. nov., sp. nov., a non-phototrophic iron-reducing bacterium closely related to phototrophic Rhodobacter species.
ジャーナル Antonie Van Leeuwenhoek
Abstract An Fe(III) oxide-reducing bacterium designated as SgZ-3(T), which could couple glucose oxidation to Fe(III) oxide reduction for energy conservation, was isolated from an electrochemical biofilm. The isolate was Gram-negative, catalase-positive and oxidase-negative, and facultative anaerobic. NaCl was not required for growth, but NaCl concentrations up to 5 % (w/v) were tolerated. Growth occurred in TSB (tryptic soy broth) at 20-40 °C (optimum 30 °C) and at pH 6.0-7.5 (optimum 7.0). Phototrophic growth could not be demonstrated. No vesicular photosynthetic membrane was observed. Bacteriochlorophyll α and carotenoids were absent. Biotin and thiamine were required as growth factors for the isolate. Phylogenetic analysis of the 16S rRNA gene sequence placed strain SgZ-3(T) within the family Rhodobacteraceae and affiliated with an phototrophic genera Rhodobacter. The G+C content of the genomic DNA was 68.6 mol%. Strain SgZ-3(T) contained Q-10 as the predominant quinone. The major cellular fatty acids were C18:1 ω6c and/or C18:1 ω7c (66.9 %) and C16:0 (9.5 %). Based on its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-3(T) (=KACC 16603(T) = CCTCC AB2012026(T)) was designated as the type strain of a new genus and a novel species of the family Rhodobacteraceae, for which the name Sinorhodobacter ferrireducens sp. nov. was proposed.
巻・号 104(5)
ページ 715-24
公開日 2013-11-1
DOI 10.1007/s10482-013-9979-0
PMID 23907520
MeSH Bacterial Typing Techniques Base Composition Catalase / metabolism Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Environmental Microbiology Fatty Acids / analysis Glucose / metabolism Hydrogen-Ion Concentration Iron / metabolism* Molecular Sequence Data Oxidation-Reduction Oxidoreductases / metabolism Phototrophic Processes Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Rhodobacteraceae / classification* Rhodobacteraceae / genetics Rhodobacteraceae / isolation & purification* Sequence Analysis, DNA Sodium Chloride / metabolism Temperature
IF 2.674
引用数 7
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 14794 JCM 14887