| RRC ID |
29437
|
| 著者 |
Sakamoto T, Otokawa T, Kono R, Shigeri Y, Watanabe K.
|
| タイトル |
A C69-family cysteine dipeptidase from Lactobacillus farciminis JCM1097 possesses strong Gly-Pro hydrolytic activity.
|
| ジャーナル |
J Biochem
|
| Abstract |
Dipeptide Gly-Pro, a hard-to-degrade and collagenous peptide, is thought to be hydrolysed by prolidases that can work on various X-Pro dipeptides. Here, we found an entirely different type of dipeptidase from Lactobacillus farciminis JCM1097 that cleaves Gly-Pro far more efficiently and with higher specificity than prolidases, and then investigated its properties by use of a recombinant enzyme. Although L. farciminis dipeptidase was expressed in the form of an inclusion body in Escherichia coli at 37 °C, it was smoothly over-expressed in a soluble form at a lower temperature. The maximal Gly-Pro hydrolytic activity was attained in E. coli at 30 °C. In contrast to prolidases that are metallopeptidases showing the modest or marginal activity toward Gly-Pro, this L. farciminis dipeptidase belongs to the cysteine peptidase family C69. Lactobacillus farciminis dipeptidase occurs in cytoplasm and utilizes the side chain of an amino-terminal cysteine residue to perform the nucleophilic attack on the target amide bond between Gly-Pro after processing eight amino acid residues at the N-terminus. Furthermore, L. farciminis dipeptidase is potent enough to synthesize Gly-Pro from Gly and Pro by a reverse reaction. These novel properties could be revealed by virtue of the success in preparing recombinant enzymes in higher yield and in a stable form.
|
| 巻・号 |
154(5)
|
| ページ |
419-27
|
| 公開日 |
2013-11-1
|
| DOI |
10.1093/jb/mvt069
|
| PII |
mvt069
|
| PMID |
23986487
|
| MeSH |
Cysteine / metabolism*
Dipeptidases / chemistry
Dipeptidases / genetics
Dipeptidases / metabolism*
Dipeptides / chemistry*
Dipeptides / metabolism*
Hydrolysis
Lactobacillus / enzymology*
Molecular Sequence Data
Sequence Alignment
Sequence Analysis, Protein
Substrate Specificity
|
| IF |
2.476
|
| 引用数 |
4
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM1097 |
