RRC ID |
29472
|
著者 |
Imura T, Kawamura D, Morita T, Sato S, Fukuoka T, Yamagata Y, Takahashi M, Wada K, Kitamoto D.
|
タイトル |
Production of sophorolipids from non-edible jatropha oil by Stamerella bombicola NBRC 10243 and evaluation of their interfacial properties.
|
ジャーナル |
J Oleo Sci
|
Abstract |
To facilitate the development of bio-based chemicals from renewable and inexpensive natural resources, we sought to produce biosurfactants using non-edible jatropha oil. Twenty yeasts known to produce biosurfactants were tested in this study, and Stamerella bombicola NBRC 10243 was found to use jatropha oil efficiently to produce sophorolipids (SLs) as a mixture of lactone-form SL (L-SL) and acid-form SL (A-SL). Under culture conditions using rice bran as the source of organic nutrients, the yield of SLs reached 122.6 g/L in 5-L jar fermentors after 9 d in culture. HPLC analysis of the culture medium showed that the levels of phorbol esters (PEs), major toxic components of the oil, decreased markedly with an increase in culture time, suggesting that the yeast degrades PEs. Although the SLs obtained by solvent extraction of the culture medium contained a small amount of PEs, the sodium salt of A-SL (A-SL-Na) obtained by alkaline treatment (5N NaOH, 80°C) showed no PE peaks upon HPLC analysis. A-SL-Na had excellent surface activity with low CMC (9.0×10⁻⁴ M) and γ(CMC) (29.6 mN/m), which are lower than that of sodium dodecyl sulfate (SDS). The solubilizing ability of A-SL-Na toward for octanoic acid ([octanoic acid]/[A-SL-Na]) was found to be 2.0, which is half that of SDS. Our findings should help improve SL production from non-edible feedstock and broaden the use of promising bio-based surfactants.
|
巻・号 |
62(10)
|
ページ |
857-64
|
公開日 |
2013-1-1
|
DOI |
10.5650/jos.62.857
|
PII |
DN/JST.JSTAGE/jos/62.857
|
PMID |
24088524
|
MeSH |
Biosynthetic Pathways*
Culture Media / chemistry
Culture Techniques
Glycolipids / biosynthesis*
Glycolipids / chemistry
Jatropha*
Microbiological Techniques
Molecular Conformation
Phorbol Esters / metabolism
Plant Oils / metabolism*
Saccharomycetales / metabolism*
Surface-Active Agents / metabolism*
Time Factors
Yeasts / metabolism
|
IF |
1.304
|
引用数 |
14
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
|
リソース情報 |
一般微生物 |
JCM 1461
JCM 10317
JCM 10318
JCM 10323
JCM 11752
JCM 16987 |