RRC ID 29472
著者 Imura T, Kawamura D, Morita T, Sato S, Fukuoka T, Yamagata Y, Takahashi M, Wada K, Kitamoto D.
タイトル Production of sophorolipids from non-edible jatropha oil by Stamerella bombicola NBRC 10243 and evaluation of their interfacial properties.
ジャーナル J Oleo Sci
Abstract To facilitate the development of bio-based chemicals from renewable and inexpensive natural resources, we sought to produce biosurfactants using non-edible jatropha oil. Twenty yeasts known to produce biosurfactants were tested in this study, and Stamerella bombicola NBRC 10243 was found to use jatropha oil efficiently to produce sophorolipids (SLs) as a mixture of lactone-form SL (L-SL) and acid-form SL (A-SL). Under culture conditions using rice bran as the source of organic nutrients, the yield of SLs reached 122.6 g/L in 5-L jar fermentors after 9 d in culture. HPLC analysis of the culture medium showed that the levels of phorbol esters (PEs), major toxic components of the oil, decreased markedly with an increase in culture time, suggesting that the yeast degrades PEs. Although the SLs obtained by solvent extraction of the culture medium contained a small amount of PEs, the sodium salt of A-SL (A-SL-Na) obtained by alkaline treatment (5N NaOH, 80°C) showed no PE peaks upon HPLC analysis. A-SL-Na had excellent surface activity with low CMC (9.0×10⁻⁴ M) and γ(CMC) (29.6 mN/m), which are lower than that of sodium dodecyl sulfate (SDS). The solubilizing ability of A-SL-Na toward for octanoic acid ([octanoic acid]/[A-SL-Na]) was found to be 2.0, which is half that of SDS. Our findings should help improve SL production from non-edible feedstock and broaden the use of promising bio-based surfactants.
巻・号 62(10)
ページ 857-64
公開日 2013-1-1
DOI 10.5650/jos.62.857
PII DN/JST.JSTAGE/jos/62.857
PMID 24088524
MeSH Biosynthetic Pathways* Culture Media / chemistry Culture Techniques Glycolipids / biosynthesis* Glycolipids / chemistry Jatropha* Microbiological Techniques Molecular Conformation Phorbol Esters / metabolism Plant Oils / metabolism* Saccharomycetales / metabolism* Surface-Active Agents / metabolism* Time Factors Yeasts / metabolism
IF 1.304
引用数 14
WOS 分野 FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED
リソース情報
一般微生物 JCM 1461 JCM 10317 JCM 10318 JCM 10323 JCM 11752 JCM 16987