| 著者 |
Zhang YG, Wang HF, Liu Q, Hozzein WN, Wadaan MA, Cheng J, Chen YJ, Zhang YM, Li WJ.
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| Abstract |
An alkaliphilic actinobacterium, designated EGI 80050(T), was isolated from a desert soil sample of Xinjiang, north-west China, and characterized by a polyphasic approach. The isolate was observed to produce purple orange-yellow aerial mycelium and dark orange-yellow substrate mycelium on yeast extract-malt extract agar medium. Whole-cell hydrolysates of strain EGI 80050(T) were found to contain LL-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose, rhamnose and mannose as the main sugars. The major fatty acids identified were C16:0-iso (36.8 %), C15:0-anteiso (17.3 %), 15:0-iso (13.2 %) and 14:0-iso (10.5 %). The predominant menaquinones detected were MK-9(H6) and MK-9(H8), while the characteristic polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylmethylethanolamine and three unknown phospholipids. The G+C content of the genomic DNA was determined to be 67.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated the strain EGI 80050(T) to the genus Streptomyces. Levels of 16 rRNA gene sequence similarities between strain EGI 80050(T) and Streptomyces candidus NRRL ISP-5141(T), Streptomyces cremeus NBRC 12760(T), Streptomyces spiroverticillatus NBRC 12821(T), Streptomyces violaceorectus NBRC 13102(T), Streptomyces cinereoruber subsp. cinereoruber NBRC 12756(T) were 96.7, 96.6, 96.6, 96.6 and 96.6 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain EGI 80050(T) is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces fukangensis sp. nov. (type strain EGI 80050(T) = BCRC 16945(T) = JCM 19127(T)) is proposed.
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