| RRC ID |
29477
|
| 著者 |
Rakshak K, Ravinder K, Nupur, Srinivas TN, Kumar PA.
|
| タイトル |
Caldimonas meghalayensis sp. nov., a novel thermophilic betaproteobacterium isolated from a hot spring of Meghalaya in northeast India.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
While studying the microbial diversity of hot springs of North-east India we isolated a strain AK31T from the Jakrem hot spring of Meghalaya. The strain formed light yellow colonies on nutrient agar and was Gram negative, non spore-forming rods, motile with single polar flagellum. The strain was positive for oxidase and catalase and hydrolysed starch and weakly urea. The predominant cellular fatty acids were C16:0 (34.8 %), C17:0 cyclo (27.1 %), C16:1 ω7c and/or iso-C15:0 2OH (summed feature 3) (9.6 %), C10:0 3OH (8.0 %), C12:0 (5.8 %), C14:0 (5.3 %) and C18:1 ω7c (5.3 %). Strain AK31T contained ubiquinone-8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and one unidentified glycolipid as the polar lipids. The G + C content of the DNA of the strain AK31T was 66.7 mol%. The 16S rRNA gene sequence analysis indicated that strain AK31T was member of the genus Caldimonas and closely related to Caldimonas manganoxidans JCM 10698T and Caldimonas taiwanensis On1T with 96.9 % similarity and with Aquincola tertiaricarbonis L10T and Azohydromonas australica IAM 12664T with 96.5 and 96.4 % similarity respectively. Phylogenetic analyses indicated that the strain AK31T clustered with C. manganoxidans JCM 10698T and C. taiwanensis On1T with a phylogenetic distance of 3.25 %. Based on data from the current polyphasic study, strain AK31T is proposed as a novel species of the genus Caldimonas, for which the name Caldimonas meghalayensis sp. nov. is proposed. The type strain of C. meghalayensis is AK31T (= MTCC 11703T = JCM 18786T).
|
| 巻・号 |
104(6)
|
| ページ |
1217-25
|
| 公開日 |
2013-12-1
|
| DOI |
10.1007/s10482-013-0043-x
|
| PMID |
24122117
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Cluster Analysis
Comamonadaceae / classification*
Comamonadaceae / genetics
Comamonadaceae / isolation & purification*
Comamonadaceae / physiology
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
Flagella / physiology
Hot Springs / microbiology*
India
Locomotion
Microscopy, Electron
Molecular Sequence Data
Phospholipids / analysis
Phylogeny
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
2.674
|
| 引用数 |
12
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 10698
JCM 18786 |
