RRC ID 2953
著者 Kaksonen AH, Spring S, Schumann P, Kroppenstedt RM, Puhakka JA.
タイトル Desulfotomaculum thermosubterraneum sp. nov., a thermophilic sulfate-reducer isolated from an underground mine located in a geothermally active area.
ジャーナル Int J Syst Evol Microbiol
Abstract A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50-72 degrees C (optimum growth at 61-66 degrees C) and the pH range was 6.4-7.8 (optimum at pH 7.2-7.4). Strain RL50JIIIT tolerated up to 1.5% NaCl, but optimum growth occurred in the presence of 0-1% NaCl. Electron acceptors utilized were sulfate, sulfite, thiosulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15:0, iso-C17:0 DMA (dimethyl acetal), iso-C15:0 DMA and iso-C17:0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7% similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA-DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).
巻・号 56(Pt 11)
ページ 2603-2608
公開日 2006-11-1
DOI 10.1099/ijs.0.64439-0
PMID 17082399
MeSH Alanine / metabolism Alcohols / metabolism Bacterial Typing Techniques Base Composition Carbohydrate Metabolism Carboxylic Acids / metabolism DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Desulfotomaculum / classification* Desulfotomaculum / cytology Desulfotomaculum / isolation & purification* Desulfotomaculum / physiology Fatty Acids / analysis Genes, rRNA Growth Inhibitors / pharmacology Hydrogen / metabolism Hydrogen-Ion Concentration Japan Lactic Acid / metabolism Mining* Molecular Sequence Data Movement Nucleic Acid Hybridization Phylogeny Pyruvic Acid / metabolism Quinones / analysis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / pharmacology Soil Microbiology* Sulfates / metabolism* Sulfites / metabolism Sulfur / metabolism Temperature
IF 2.415
引用数 35
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 13837?