論文 - 詳細
| RRC ID | 2955 |
|---|---|
| 著者 | Keszthelyi A, Ohkusu M, Takeo K, Pfeiffer I, Litter J, Kucsera J. |
| タイトル | Characterisation of the anticryptococcal effect of the FC-1 toxin produced by Filobasidium capsuligenum. |
| ジャーナル | Mycoses |
| Abstract |
The basidiomycetous yeast Filobasidium capsuligenum produces a killer toxin (FC-1) which is highly effective against the opportunistic fungal pathogen Cryptococcus neoformans. The aim of this work was to study the effect of the toxin on C. neoformans cells. The sensitivities of strains representing eight molecular subtypes (VNI-IV and VGI-IV) of the C. neoformans species complex, and of an additional 50 clinical and environmental isolates were determined. Analysis of cellular DNA by laser scanning cytometry and fluorescein isothiocyanate (FITC) staining of the toxin-treated cells revealed that the killing mechanism of FC-1 is neither cell cycle- nor cell wall biosynthesis-dependent; rather it may act as an ionophoric protein that disrupts the cytoplasmic membrane function. The competition assay results suggest that beta-1,6-glucan in the cell wall may provide the binding site for the killer protein. This anticryptococcal toxin has the potential to be applied as a therapeutic agent for the treatment of cryptococcosis. |
| 巻・号 | 49(3) |
| ページ | 176-83 |
| 公開日 | 2006-5-1 |
| DOI | 10.1111/j.1439-0507.2006.01227.x |
| PII | MYC1227 |
| PMID | 16681807 |
| MeSH | Animals Basidiomycota / growth & development Basidiomycota / metabolism* Cell Membrane / drug effects* Cryptococcus neoformans / classification Cryptococcus neoformans / drug effects* Cryptococcus neoformans / genetics Culture Media Humans Killer Factors, Yeast Mycotoxins / metabolism Mycotoxins / pharmacology* Species Specificity |
| IF | 3.575 |
| 引用数 | 11 |
| WOS 分野 | DERMATOLOGY MYCOLOGY |
| リソース情報 | |
| 病原微生物 | IFM 5844 IFM 5845 IFM 5860 IFM 40009 IFM 40022 IFM 40038 IFM 40078 IFM 40190 IFM 45940 IFM 46816 IFM 46823 IFM 46829 IFM 46834 IFM 46843 IFM 47840 IFM 47845 IFM 47848 IFM 47853 IFM 47854 IFM 47871 IFM 49120 IFM 49123 IFM 49138 IFM 49142 IFM 49144 IFM 49146 IFM 49149 IFM 49152 IFM 49161 IFM 49171 IFM 49175 IFM 49177 IFM 49181 IFM 49186 IFM 50750 IFM 50752 IFM 50753 IFM 50754 IFM 50764 IFM 50765 IFM 51676 IFM 51680 IFM 51688 IFM 51693 IFM 51700 IFM 51701 IFM 51702 IFM 51703 IFM 51704 IFM 51705 IFM 51706 IFM 51708 IFM 51709 IFM 51710 IFM 51711 JCM 1532 JCM 1376 JCM 1461 |