論文 - 詳細
| RRC ID | 29629 |
|---|---|
| 著者 | Kakimoto M, Ishikawa T, Miyagi A, Saito K, Miyazaki M, Asaeda T, Yamaguchi M, Uchimiya H, Kawai-Yamada M. |
| タイトル | Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata. |
| ジャーナル | J Plant Physiol |
| Abstract |
A volatile metabolite, 2-methylisoborneol (2-MIB), causes an unpleasant taste and odor in tap water. Some filamentous cyanobacteria produce 2-MIB via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). We isolated the genes encoding GPPMT and MIBS from Pseudanabaena galeata, a filamentous cyanobacterium known to be a major causal organism of 2-MIB production in Japanese lakes. The predicted amino acid sequence showed high similarity with that of Pseudanabaena limnetica (96% identity in GPPMT and 97% identity in MIBS). P. galeata was cultured at different temperatures to examine the effect of growth conditions on the production of 2-MIB and major metabolites. Gas chromatograph-mass spectrometry (GC-MS) measurements showed higher accumulation of 2-MIB at 30 °C than at 4 °C or 20 °C after 24 h of culture. Real-time-RT PCR analysis showed that the expression levels of the genes encoding GPPMT and MIBS decreased at 4 °C and increased at 30 °C, compared with at 20 °C. Furthermore, metabolite analysis showed dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures. |
| 巻・号 | 171(3-4) |
| ページ | 292-300 |
| 公開日 | 2014-2-15 |
| DOI | 10.1016/j.jplph.2013.09.005 |
| PII | S0176-1617(13)00360-X |
| PMID | 24140001 |
| MeSH | Camphanes / metabolism Cyanobacteria / genetics* Cyanobacteria / metabolism* Gene Expression Regulation, Bacterial* Metabolic Networks and Pathways / physiology* Temperature* |
| IF | 3.013 |
| 引用数 | 18 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 藻類 | NIES-512 |