論文 - 詳細
| RRC ID | 29653 |
|---|---|
| 著者 | Sakaguchi M, Murakami H, Suzaki T. |
| タイトル | Involvement of a 40-kDa glycoprotein in food recognition, prey capture, and induction of phagocytosis in the protozoon Actinophrys sol. |
| ジャーナル | Protist |
| Abstract |
A 40-kDa glycoprotein (gp40) was identified as a Con A-binding adhesive substance of the heliozoon Actinophrys sol for immobilizing and ingesting prey flagellates. Isolation and partial characterization of gp40 showed that: 1) gp40 is a major Con A-binding protein of Actinophrys with a molecular weight of 40 kDa, and is stored in secretory granules called extrusomes; 2) gp40 was purified by Con A-affinity chromatography, and the N-terminal amino acid sequence was determined as H2N-KVLK-FEDDFDTFDLQ; 3) prey flagellates became adhered to gp40-immobilized agarose beads; 4) phagocytosis of Actinophrys was induced against gp40-immobilized agarose beads; and 5) solubilized gp40 induced exocytosis of extrusomes and cell fusion of heliozoons. These results indicate that gp40 is a multi-functional secretory protein of Actinophrys, which is required in correct targeting of the heliozoon to food organisms as well as in self-recognition. |
| 巻・号 | 152(1) |
| ページ | 33-41 |
| 公開日 | 2001-5-1 |
| DOI | 10.1078/1434-4610-00041 |
| PII | S1434-4610(04)70041-3 |
| PMID | 11401035 |
| MeSH | Amino Acid Sequence Animals Chromatography, Affinity Concanavalin A Eukaryota / physiology* Glycoproteins / chemistry Glycoproteins / isolation & purification Glycoproteins / physiology* Molecular Sequence Data Molecular Weight Peptide Fragments / chemistry Phagocytosis* Predatory Behavior Protozoan Proteins / chemistry Protozoan Proteins / isolation & purification Protozoan Proteins / physiology* Secretory Vesicles / physiology |
| IF | 1.989 |
| 引用数 | 25 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 藻類 | NIES-2497 |