Reference - Detail
| RRC ID | 29676 |
|---|---|
| Author | Jiménez-Hidalgo M, Kurz CL, Pedrajas JR, Naranjo-Galindo FJ, González-Barrios M, Cabello J, Sáez AG, Lozano E, Button EL, Veal EA, Fierro-González JC, Swoboda P, Miranda-Vizuete A. |
| Title | Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin. |
| Journal | Free Radic Biol Med |
| Abstract |
Thioredoxins are a class of evolutionarily conserved proteins that have been demonstrated to play a key role in many cellular processes involving redox reactions. We report here the genetic and biochemical characterization of Caenorhabditis elegans TRX-3, the first metazoan thioredoxin with an intestine-specific expression pattern. By using green fluorescent protein reporters we have found that TRX-3 is expressed in both the cytoplasm and the nucleus of intestinal cells, with a prominent localization at the apical membrane. Although intestinal function, reproductive capacity, longevity, and resistance of trx-3 loss-of-function mutants to many stresses are indistinguishable from those of wild-type animals, we have observed a slight reduction in size and a minor reduction in the defecation cycle timing of trx-3 mutants. Interestingly, trx-3 is induced upon infection by Photorhabdus luminescens and Candida albicans, and TRX-3 overexpression provides a modest protection against these pathogens. Together, our data indicate that TRX-3 function in the intestine is dispensable for C. elegans development but may be important to fight specific bacterial and fungal infections. |
| Volume | 68(100) |
| Pages | 205-19 |
| Published | 2014-3-1 |
| DOI | 10.1016/j.freeradbiomed.2013.11.023 |
| PII | S0891-5849(13)01528-1 |
| PMID | 24316195 |
| PMC | PMC4018987 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Intestinal Mucosa / metabolism* Longevity / genetics Organ Specificity Oxidation-Reduction Thioredoxins / biosynthesis* Thioredoxins / genetics |
| IF | 6.17 |
| Times Cited | 9 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY ENDOCRINOLOGY & METABOLISM |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2820 |