RRC ID 29681
Author Tsubota T, Uchino K, Kamimura M, Ishikawa M, Hamamoto H, Sekimizu K, Sezutsu H.
Title Establishment of transgenic silkworms expressing GAL4 specifically in the haemocyte oenocytoid cells.
Journal Insect Mol Biol
Abstract Insect haemocytes play significant roles in innate immunity. The silkworm, a lepidopteran species, is often selected as the model for studies into the functions of haemocytes in immunity; however, our understanding of the role of haemocytes remains limited because the lack of haemocyte promoters for transgene expression makes genetic manipulations difficult. In the present study, we aimed to establish transgenic silkworm strains expressing GAL4 in their haemocytes. First, we identified three genes with strong expression in haemocytes, namely, lp44, Haemocyte Protease 1 (HP1) and hemocytin. Transgenic silkworms expressing GAL4 under the control of the putative promoters of these genes were then established and expression was examined. Although GAL4 expression was not detected in haemocytes of HP1-GAL4 or hemocytin-GAL4 strains, lp44-GAL4 exhibited a high level of GAL4 expression, particularly in oenocytoids. GAL4 expression was also detected in the midgut but in no other tissues, indicating that GAL4 expression in this strain is mostly oenocytoid-specific. Thus, we have identified a promoter that enables oenocytoid expression of genes of interest. Additionally, the lp44-GAL4 strain could also be used for other types of research, such as the functional analysis of genes in oenocytoids, which would facilitate advances in our understanding of insect immunity.
Volume 23(2)
Pages 165-74
Published 2014-4-1
DOI 10.1111/imb.12071
PMID 24237591
MeSH Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / immunology Animals, Genetically Modified / metabolism Bombyx / genetics* Bombyx / growth & development Bombyx / immunology Bombyx / metabolism DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Gene Expression Regulation* Hemocytes / metabolism* Immunity, Innate Insect Proteins / genetics* Insect Proteins / metabolism Larva / genetics Larva / immunology Larva / metabolism Lectins / genetics Lectins / metabolism Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism Transcription Factors / genetics* Transcription Factors / metabolism
IF 2.437
Times Cited 7
WOS Category ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Silkworms