| RRC ID |
29683
|
| 著者 |
Ohno K, Sawada J, Takiya S, Kimoto M, Matsumoto A, Tsubota T, Uchino K, Hui CC, Sezutsu H, Handa H, Suzuki Y.
|
| タイトル |
Silk gland factor-2, involved in fibroin gene transcription, consists of LIM homeodomain, LIM-interacting, and single-stranded DNA-binding proteins.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
SGF-2 binds to promoter elements governing posterior silk gland-specific expression of the fibroin gene in Bombyx mori. We purified SGF-2 and showed that SGF-2 contains at least four gene products: the silkworm orthologues of LIM homeodomain protein Awh, LIM domain-binding protein (Ldb), a sequence-specific single-stranded DNA-binding protein (Lcaf), and the silk protein P25/fibrohexamerin (fhx). Using co-expression of these factors in Sf9 cells, Awh, Ldb, and Lcaf proteins were co-purified as a ternary complex that bound to the enhancer sequence in vitro. Lcaf interacts with Ldb as well as Awh through the conserved regions to mediate transcriptional activation in yeast. Misexpression of Awh in transgenic silkworms induces ectopic expression of the fibroin gene in the middle silk glands, where Ldb and Lcaf are expressed. Taken together, this study demonstrates that SGF-2 is a multisubunit activator complex containing Awh. Moreover, our results suggest that the Ldb·Lcaf protein complex serves as a scaffold to facilitate communication between transcriptional control elements.
|
| 巻・号 |
288(44)
|
| ページ |
31581-91
|
| 公開日 |
2013-11-1
|
| DOI |
10.1074/jbc.M113.514471
|
| PII |
S0021-9258(20)48632-6
|
| PMID |
24022586
|
| PMC |
PMC3814754
|
| MeSH |
Amino Acid Sequence
Animals
Bombyx / genetics
Bombyx / metabolism*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Fibroins / biosynthesis*
Fibroins / genetics
LIM-Homeodomain Proteins / genetics
LIM-Homeodomain Proteins / metabolism*
Molecular Sequence Data
Response Elements / physiology
Sf9 Cells
Spodoptera
Trans-Activators / genetics
Trans-Activators / metabolism*
Transcription, Genetic / physiology*
|
| IF |
4.238
|
| 引用数 |
23
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
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| リソース情報 |
| カイコ |
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