| RRC ID |
29749
|
| Author |
Ohtsubo E, Hsu MT.
|
| Title |
Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB.
|
| Journal |
J Bacteriol
|
| Abstract |
The genetic and physical structures of commonly used F-prime factors carrying the galactose region of the Escherichia coli chromosome were analyzed. Deletions in the chromosomal DNA sequences in the F-prime factors were found to be frequent events. A genetic method was developed to reconstruct the original F-prime factors from deletion variants. Heteroduplex analysis of the reconstructed F-prime factors confirmed the derivation of the F-prime factors F100 and F152, from the same Hfr, and finally determined the normal E. coli chromosomal sequence in the region between fep and uvrB, containing about 5 min in genetic units and about 246.5 in kilobase units (kb). This sequence could be connected with the DNA sequences of the lac-purE region, which had been physically determined previously. Together they constituted a total of 528.6 kb. From these combined sequences, the distance from lacPO to galK was calculated to be 412.9 kb, which corresponds to 8.8 min in genetic units.
|
| Volume |
134(3)
|
| Pages |
778-94
|
| Published |
1978-6-1
|
| DOI |
10.1128/jb.134.3.778-794.1978
|
| PMID |
350847
|
| PMC |
PMC222324
|
| MeSH |
Chromosome Mapping
Chromosomes, Bacterial*
DNA, Bacterial / analysis
DNA, Bacterial / genetics*
Escherichia coli / genetics*
Escherichia coli / ultrastructure
F Factor*
Microscopy, Electron
Molecular Weight
|
| IF |
3.006
|
| Resource |
| Prokaryotes E. coli |
ME8619 |
