| RRC ID |
29753
|
| Author |
Sugimoto K, Oka A, Sugisaki H, Takanami M, Nishimura A, Yasuda Y, Hirota Y.
|
| Title |
Nucleotide sequence of Escherichia coli K-12 replication origin.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
From subfragments of an EcoRI fragment (9 kilobase pairs) that contained the replication origin of the Escherichia coli chromosome and had been cloned as a recombinant with a nonreplicating DNA fragment coding for ampicillin resistance, small derivative plasmids were constructed. The smallest of these, pTSO151, contained a segment of 463 base pairs as the chromosomal component. Another plasmid, pSY134, constructed from BamHI digests of the EcoRI fragment and mini-F(pMF21), contained a region of 422 base pairs identical with a corresponding region in pTSO151. We conclude that the replication origin of E. coli chromosome is located within this 422-base-pair segment. The nucleotide sequence of this segment is presented.
|
| Volume |
76(2)
|
| Pages |
575-9
|
| Published |
1979-2-1
|
| DOI |
10.1073/pnas.76.2.575
|
| PMID |
370832
|
| PMC |
PMC382991
|
| MeSH |
Base Sequence
DNA Replication*
DNA Restriction Enzymes / metabolism
DNA, Bacterial / genetics*
Escherichia coli / genetics*
Plasmids
|
| IF |
9.412
|
| Resource |
| Prokaryotes E. coli |
JE6702
JE6703
JE6705 |
