Reference - Detail
| RRC ID | 29753 |
|---|---|
| Author | Sugimoto K, Oka A, Sugisaki H, Takanami M, Nishimura A, Yasuda Y, Hirota Y. |
| Title | Nucleotide sequence of Escherichia coli K-12 replication origin. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
From subfragments of an EcoRI fragment (9 kilobase pairs) that contained the replication origin of the Escherichia coli chromosome and had been cloned as a recombinant with a nonreplicating DNA fragment coding for ampicillin resistance, small derivative plasmids were constructed. The smallest of these, pTSO151, contained a segment of 463 base pairs as the chromosomal component. Another plasmid, pSY134, constructed from BamHI digests of the EcoRI fragment and mini-F(pMF21), contained a region of 422 base pairs identical with a corresponding region in pTSO151. We conclude that the replication origin of E. coli chromosome is located within this 422-base-pair segment. The nucleotide sequence of this segment is presented. |
| Volume | 76(2) |
| Pages | 575-9 |
| Published | 1979-2-1 |
| DOI | 10.1073/pnas.76.2.575 |
| PMID | 370832 |
| PMC | PMC382991 |
| MeSH | Base Sequence DNA Replication* DNA Restriction Enzymes / metabolism DNA, Bacterial / genetics* Escherichia coli / genetics* Plasmids |
| IF | 9.412 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | JE6702 JE6703 JE6705 |