論文 - 詳細
| RRC ID | 29758 |
|---|---|
| 著者 | Nakamura K, Inouye M. |
| タイトル | DNA sequence of the gene for the outer membrane lipoprotein of E. coli: an extremely AT-rich promoter. |
| ジャーナル | Cell |
| Abstract |
The outer membrane lipoprotein is the most abundant protein in an E. coli cell. Its structural gene (Ipp) was cloned into a lambda phage vector and the nucleotide sequence of a DNA fragment of 814 bp encompassing the Ipp gene was determined. The promoter region of the gene was found to have the following features. First, a segment of 261 bp preceding the transcription initiation site (-1 to -261) has a very high AT content of 70%, in contrast to 53% for the mRNA region of 322 bp, 44% for a segment of 127 bp after the transcription termination site and 49% for the average AT content of the E. coli chromosome. Second, in particular, of the first 45 bp upstream from the transcription initiation site (-1 to -45), 36 bases (80%) are A or T. Third, there is a heptanucleotide sequence homologous to the "Pribnow box," eight bases apart from the transcription initiation site. Fourth, a sequence homologous to the "RNA polymerase recognition site" exists on both strands between positions -27 and -39. Finally, there is a long dyad symmetry centered at the transcription initiation site. |
| 巻・号 | 18(4) |
| ページ | 1109-17 |
| 公開日 | 1979-12-1 |
| DOI | 10.1016/0092-8674(79)90224-1 |
| PII | 0092-8674(79)90224-1 |
| PMID | 391404 |
| MeSH | Adenine / analysis Bacterial Proteins / genetics* Base Sequence Cloning, Molecular DNA, Bacterial / analysis DNA, Bacterial / genetics* Escherichia coli / genetics* Lipoproteins / genetics* Membrane Proteins / genetics* Operon* Thymine / analysis Transcription, Genetic |
| IF | 38.637 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 32 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME8987 |