Reference - Detail
| RRC ID | 29792 |
|---|---|
| Author | Fujita Y, Shindo K, Miwa Y, Yoshida K. |
| Title | Bacillus subtilis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli. |
| Journal | Gene |
| Abstract |
The Bacillus subtilis inositol dehydrogenase (Idh)-encoding gene (idh) was cloned in the B. subtilis temperate phage, rho 11, and then in Escherichia coli plasmids (pBR322 and pUC118). The nucleotide sequence of the idh gene, which consists of 344 codons and whose product has an Mr of 38,351, was determined. E. coli, bearing pIOL05d15, in which expression of the idh gene is under the control of the lac promoter of pUC118, overproduced an active Idh to approx. 20% of total protein upon addition of isopropyl-beta-D-thiogalactopyranoside. This overproduced enzyme cross-reacted with an anti-Idh antibody, and exhibited the same Mr and substrate specificity as those of the B. subtilis enzyme. |
| Volume | 108(1) |
| Pages | 121-5 |
| Published | 1991-12-1 |
| DOI | 10.1016/0378-1119(91)90496-x |
| PII | 0378-1119(91)90496-X |
| PMID | 1761221 |
| MeSH | Amino Acid Sequence Bacillus subtilis / enzymology* Bacillus subtilis / genetics Base Sequence Blotting, Western Cloning, Molecular DNA Mutational Analysis Escherichia coli / genetics Escherichia coli / metabolism Gene Expression / genetics Genes, Bacterial Molecular Sequence Data Sugar Alcohol Dehydrogenases / genetics* |
| IF | 2.984 |
| Times Cited | 28 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | ME9631 ME9632 ME9633 |
| Prokaryotes B. subtilis | MBS92 MBS149 |