RRC ID 29831
Author Russel M, Model P.
Title Direct cloning of the trxB gene that encodes thioredoxin reductase.
Journal J Bacteriol
Abstract A strain was constructed which contains mutations in the genes encoding thioredoxin (trxA) and thioredoxin reductase (trxB) such that filamentous phage f1 cannot grow. The complementation of either mutation with its wild-type allele permits phage growth. We used this strain to select f1 phage which contain a cloned trxB gene. The location of the gene on the cloned fragment was determined, and its protein product was identified. Plasmid subclones that contain this gene overproduce thioredoxin reductase.
Volume 163(1)
Pages 238-42
Published 1985-7-1
DOI 10.1128/JB.163.1.238-242.1985
PMID 2989245
PMC PMC219103
MeSH Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial / genetics Escherichia coli / genetics* Gene Expression Regulation Genes, Bacterial* NADH, NADPH Oxidoreductases / genetics* Plasmids Thioredoxin-Disulfide Reductase / genetics* Transcription, Genetic
IF 3.234
Times Cited 86
Prokaryotes E. coli ME8446