論文 - 詳細
| RRC ID | 29831 |
|---|---|
| 著者 | Russel M, Model P. |
| タイトル | Direct cloning of the trxB gene that encodes thioredoxin reductase. |
| ジャーナル | J Bacteriol |
| Abstract |
A strain was constructed which contains mutations in the genes encoding thioredoxin (trxA) and thioredoxin reductase (trxB) such that filamentous phage f1 cannot grow. The complementation of either mutation with its wild-type allele permits phage growth. We used this strain to select f1 phage which contain a cloned trxB gene. The location of the gene on the cloned fragment was determined, and its protein product was identified. Plasmid subclones that contain this gene overproduce thioredoxin reductase. |
| 巻・号 | 163(1) |
| ページ | 238-42 |
| 公開日 | 1985-7-1 |
| DOI | 10.1128/jb.163.1.238-242.1985 |
| PMID | 2989245 |
| PMC | PMC219103 |
| MeSH | Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial / genetics Escherichia coli / genetics* Gene Expression Regulation Genes, Bacterial* NADH, NADPH Oxidoreductases / genetics* Plasmids Thioredoxin-Disulfide Reductase / genetics* Transcription, Genetic |
| IF | 3.006 |
| 引用数 | 86 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME8446 |